ArticlePLoS pathogens2019
An autoinducer-independent RhlR quorum-sensing receptor enables analysis of RhlR regulation.
Article in PLoS pathogens, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 44 citations in OpenAlex.
- Natural products shape microbial ecology and disease in the cystic fibrosis airway.Natural product reports · 2026Review
- MrtR ofProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Microbial quorum sensing signals, symbiosis, secrets and sabotage.Microbiology (Reading, England) · 2026Review
- The conserved coordination of acyl-homoserine lactone and PqsE signaling defines the RhlR-dependent quorum-sensing network inbioRxiv : the preprint server for biology · 2026Article
- RhlR quorum-sensing receptor ligand sensitivity regulates the differential expression of phenazine genes inJournal of bacteriology · 2026Article
- Phytochemical Quorum-Sensing Inhibitors Against Bacterial Pathogens: Mechanisms of Action and Translational Challenges.Current issues in molecular biology · 2026Review
- Co-Occurrence of Resistance to Aminoglycosides and Carbapenems inInternational journal of microbiology · 2026Review
- Dickeya zeae WH1 as sensor for cost-effective detection of pyocyanin produced by Pseudomonas aeruginosa.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Natural product-based inhibitors of quorum sensing: A novel approach to combat antibiotic resistance.Biochemistry and biophysics reports · 2025Review
- Regulatory roles of an sRNA derived from the 5´ UTR and sequence internal toMicrobiology spectrum · 2025Article
- c-di-GMP phosphodiesterase ProE interacts with quorum sensing protein PqsE to promote pyocyanin production inmSphere · 2025Article
- Reprofiling lamivudine as an antibiofilm and anti-pathogenic agent against Pseudomonas aeruginosa.AMB Express · 2025Article
- Quorum Sensing and its Inhibition inCurrent drug targets · 2025Review
- A comprehensive review of the pathogenic mechanisms ofFrontiers in microbiology · 2025Review
- Effects of elevated levels of intracellular nitric oxide on Pseudomonas aeruginosa biofilm in chronic skin wound and slow-killing infection models.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Citrate cross-feeding bymBio · 2024Article
- Article
- Using next generation antimicrobials to target the mechanisms of infection.npj antimicrobials and resistance · 2023Review
- Structure of the RhlR-PqsE complex from Pseudomonas aeruginosa reveals mechanistic insights into quorum-sensing gene regulation.Structure (London, England : 1993) · 2022Article
- Moonlighting chaperone activity of the enzyme PqsE contributes to RhlR-controlled virulence of Pseudomonas aeruginosa.Nature communications · 2022Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
Quorum sensing is a chemical communication process that bacteria use to coordinate group behaviors. Pseudomonas aeruginosa, an opportunistic pathogen, employs multiple quorum-sensing systems to control behaviors including virulence factor production and biofilm formation. One P. aeruginosa quorum-sensing receptor, called RhlR, binds the cognate autoinducer N-butryl-homoserine lactone (C4HSL), and the RhlR:C4HSL complex activates transcription of target quorum-sensing genes. Here, we use a genetic screen to identify RhlR mutants that function independently of the autoinducer. The RhlR Y64F W68F V133F triple mutant, which we call RhlR*, exhibits ligand-independent activity in vitro and in vivo. RhlR* can drive wildtype biofilm formation and infection in a nematode animal model. The ability of RhlR* to properly regulate quorum-sensing-controlled genes in vivo depends on the quorum-sensing regulator RsaL keeping RhlR* activity in check. RhlR is known to function together with PqsE to control production of the virulence factor called pyocyanin. Likewise, RhlR* requires PqsE for pyocyanin production in planktonic cultures, however, PqsE is dispensable for RhlR*-driven pyocyanin production on surfaces. Finally, wildtype RhlR protein is not sufficiently stabilized by C4HSL to allow purification. However, wildtype RhlR can be stabilized by the synthetic ligand mBTL (meta-bromo-thiolactone) and RhlR* is stable without a ligand. These features enabled purification of the RhlR:mBTL complex and of RhlR* for in vitro examination of their biochemical activities. To our knowledge, this work reports the first RhlR protein purification.
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