Data on the target search by a single protein on DNA measured with ultrafast force-clamp spectroscopy.

Carina Monico, Alessia Tempestini, Lucia Gardini, Francesco Saverio Pavone, Marco Capitanio

Abstract read

In one paragraph

Article in Data in brief, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Data on the target search by a single protein on DNA measured with ultrafast force-clamp spectroscopy.Data in brief · 2019
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Carina MonicoLENS - European Laboratory for Non-linear Spectroscopy, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy.

Alessia TempestiniLENS - European Laboratory for Non-linear Spectroscopy, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy.

Lucia GardiniLENS - European Laboratory for Non-linear Spectroscopy, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy.

Francesco Saverio PavoneLENS - European Laboratory for Non-linear Spectroscopy, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy.

Marco CapitanioLENS - European Laboratory for Non-linear Spectroscopy, Via Nello Carrara 1, 50019, Sesto Fiorentino, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The mechanism by which proteins are able to find small cognate sequences in the range from few to few tens of base pairs amongst the millions of non-specific chromosomal DNA has been puzzling researchers for decades. Single molecule techniques based on fluorescence have been successfully applied to investigate this process but are inherently limited in terms of spatial and temporal resolution. We previously showed that ultrafast force-clamp spectroscopy, a single molecule technique based on laser tweezers, can be applied to the study of protein-DNA interaction attaining sub-millisecond and few base-pair resolution. Here, we share experimental records of interactions between a single lactose repressor protein and DNA collected under different forces using our technique [1]. The data can be valuable for researchers interested in the study of protein-DNA interaction and the mechanism of DNA target search, both from an experimental and modeling point of view. The data is related to the research article "

Indexed as

DNAForce-clamp spectroscopyLactose repressorOptical tweezersSingle molecule biophysics

Identifiers

PMID31193354

DOI10.1016/j.dib.2019.103918

PMCPMC6526245

What OpenQuestion holds

Textmetadata

LicenceCC BY-NC-ND

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.