ArticleChemical science2019
A simple, robust, universal assay for real-time enzyme monitoring by signalling changes in nucleoside phosphate anion concentration using a europium(iii)-based anion receptor.
Article in Chemical science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Advances in lanthanide probes for selective anion recognition and sensing in water.Chemical science · 2026Review
- Multiple-Use Microplate Assay for Submicromolar Ultra High-Throughput Separation of Amines Based on their Degree of Substitution.ChemistryOpen · 2026Article
- Steric and Geometric Tuning of π-Conjugated Antennae in Europium(III) Complexes for Selective ADP Recognition.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Elucidating Polyphosphate Anion Binding to Lanthanide Complexes Using EXAFS and Pulsed EPR Spectroscopy.Inorganic chemistry · 2024Article
- Monitoring Enzyme Activity Using Near-Infrared Fluorescent Single-Walled Carbon Nanotubes.ACS sensors · 2024Review
- Design of a chimeric glycosyltransferase OleD for the site-specific O-monoglycosylation of 3-hydroxypyridine in nosiheptide.Microbial biotechnology · 2023Article
- Expedient synthesis and luminescence sensing of the inositol pyrophosphate cellular messenger 5-PP-InsPChemical science · 2023Article
- Anion-Driven Circularly Polarized Luminescence Inversion of Unsymmetrical Europium(III) Complexes for Target Identifiable Sensing.Inorganic chemistry · 2022Article
- Luminescent Lanthanide Probes for Inorganic and Organic Phosphates.Chemistry, an Asian journal · 2022Review
- Sterically demanding macrocyclic Eu(iii) complexes for selective recognition of phosphate and real-time monitoring of enzymatically generated adenosine monophosphate.Chemical science · 2022Article
- Anion binding to a cationic europium(III) probe enables the first real-time assay of heparan sulfotransferase activity.Organic & biomolecular chemistry · 2022Article
- A Chiral Lanthanide Tag for Stable and Rigid Attachment to Single Cysteine Residues in Proteins for NMR, EPR and Time-Resolved Luminescence Studies.Chemistry (Weinheim an der Bergstrasse, Germany) · 2021Article
- Squaramide-Based Self-Associating Amphiphiles for Anion Recognition.ChemPlusChem · 2021Article
- Versatile small molecule kinase assay through real-time, ratiometric fluorescence changes based on a pyrene-DPA-ZnRSC advances · 2021Article
- Advances in anion binding and sensing using luminescent lanthanide complexes.Chemical science · 2021Review
- HOCl Responsive Lanthanide Complexes Using Hydroquinone Caging Units.Molecules (Basel, Switzerland) · 2020Article
- Tuning the anion binding properties of lanthanide receptors to discriminate nucleoside phosphates in a sensing array.Chemical science · 2020Article
- Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe.Chemical science · 2020Article
- Label-Free Fluorescent Kinase and Phosphatase Enzyme Assays with Supramolecular Host-Dye Pairs.ChemistryOpen · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Enzymes that consume and produce nucleoside polyphosphate (NPP) anions represent major targets in drug discovery. For example, protein kinases are one of the largest classes of drug targets in the fight against cancer. The accurate determination of enzyme kinetics and mechanisms is a critical aspect of drug discovery research. To increase confidence in the selection of lead drug compounds it is crucial that pharmaceutical researchers have robust, affordable assays to measure enzyme activity accurately. We present a simple, sensitive microplate assay for real-time monitoring of a range of pharmaceutically important enzyme reactions that generate NPP anions, including kinases and glycosyltransferases. Our assay utilises a single, stable europium(iii) complex that binds reversibly to NPP anions, signalling the dynamic changes in NPP product/substrate ratio during an enzyme reaction using time-resolved luminescence. This supramolecular approach to enzyme monitoring overcomes significant limitations in existing assays, obviating the need for expensive antibodies or equipment, chemically labelled substrates or products and isolation or purification steps. Our label and antibody-free method enables rapid and quantitative analysis of enzyme activities and inhibition, offering a potentially powerful tool for use in drug discovery, suitable for high-throughput screening of inhibitors and accurate measurements of enzyme kinetic parameters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.