ArticleStem cells international2019
Generation and miRNA Characterization of Equine Induced Pluripotent Stem Cells Derived from Fetal and Adult Multipotent Tissues.
Article in Stem cells international, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Selective oncolytic activity of newcastle disease virus in equine sarcoid primary cells: a preclinical investigation.In vitro cellular & developmental biology. Animal · 2026Article
- Derivation of Equine Mesenchymal Stem/Stromal Cells from Induced Pluripotent Stem Cells via the Neural Crest Pathway and Characterisation by Immunophenotype and Tri-Lineage Differentiation.Animals : an open access journal from MDPI · 2026Article
- Generation of equine induced pluripotent stem cells from cells of embryonic, perinatal and adult tissues.Stem cell research & therapy · 2025Article
- Recent advances of miR-23 in human diseases and growth development.Non-coding RNA research · 2025Review
- Generation of bovine iPSCs from fetal fibroblasts for in vitro myogenesis and cultured meat.Frontiers in nutrition · 2025Article
- Emerging Contributions of Pluripotent Stem Cells to Reproductive Technologies in Veterinary Medicine.Journal of developmental biology · 2024Review
- The impact of induced pluripotent stem cells in animal conservation.Veterinary research communications · 2024Review
- The occurrence and development of induced pluripotent stem cells.Frontiers in genetics · 2024Review
- Exogenous OCT4 and SOX2 Contribution to In Vitro Reprogramming in Cattle.Biomedicines · 2023Article
- Reprogramming efficiency and pluripotency of mule iPSCs over its parents†.Biology of reproduction · 2023Article
- Influence of Cell Type in In Vitro Induced Reprogramming in Cattle.Life (Basel, Switzerland) · 2022Article
- Article
- iPSC Therapy for Myocardial Infarction in Large Animal Models: Land of Hope and Dreams.Biomedicines · 2021Review
- Neural Derivates of Canine Induced Pluripotent Stem Cells-Like Cells From a Mild Cognitive Impairment Dog.Frontiers in veterinary science · 2021Article
- Induced pluripotent stem cells from farm animals.Journal of animal science · 2020Review
- Generation of induced pluripotent stem cells from large domestic animals.Stem cell research & therapy · 2020Article
- In Vitro Induction of Pluripotency from Equine Fibroblasts in 20% or 5% Oxygen.Stem cells international · 2020Article
- Induced pluripotent stem cells throughout the animal kingdom: Availability and applications.World journal of stem cells · 2019Review
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionPluripotent stem cells are believed to have greater clinical potential than mesenchymal stem cells due to their ability to differentiate into almost any cell type of an organism, and since 2006, the generation of patient-specific induced pluripotent stem cells (iPSCs) has become possible in multiple species.
objectivesWe hypothesize that different cell types respond differently to the reprogramming process; thus, the goals of this study were to isolate and characterize equine adult and fetal cells and induce these cells to pluripotency for future regenerative and translational purposes.
methodsAdult equine fibroblasts (eFibros) and mesenchymal cells derived from the bone marrow (eBMmsc), adipose tissue (eADmsc), and umbilical cord tissue (eUCmsc) were isolated, their multipotency was characterized, and the cells were induced
resultsMultipotent cells were successfully isolated, but the eBMmsc failed to generate eiPSCs. The eADmsc-, eUCmsc-, and eFibros-derived iPSCs were positive for alkaline phosphatase, OCT4 and NANOG, were exclusively dependent on bFGF, and formed embryoid bodies. The miRNA profile revealed a segregated pattern between the eiPSCs and multipotent controls: the levels of miR-302/367 and the miR-92 family were increased in the eiPSCs, while the levels of miR-23, miR-27, and miR-30, as well as the let-7 family were increased in the nonpluripotent cells.
conclusionsWe were able to generate bFGF-dependent iPSCs from eADmsc, eUCmsc, and eFibros with human OSKM, and the miRNA profile revealed that clonal lines may respond differently to the reprogramming process.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.