Evidence map›Paper›PMID 31191544›Full record

ArticleFrontiers in immunology2019

Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation.

Manami Igata, Md Aminul Islam, Asuka Tada, Michihiro Takagi, A K M Humayun Kober, Leonardo Albarracin, Hisashi Aso, Wakako Ikeda-Ohtsubo, Kenji Miyazawa, Kazutoyo Yoda and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in immunology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.7field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 3 countries.

Manami IgataFood and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Md Aminul IslamFood and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Asuka TadaFood and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Michihiro TakagiFood and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
A K M Humayun KoberFood and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Leonardo AlbarracinFood and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Hisashi AsoLivestock Immunology Unit, International Education and Research Centre for Food and Agricultural Immunology (CFAI), Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Wakako Ikeda-OhtsuboFood and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Kenji MiyazawaTechnical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama, Japan.
Kazutoyo YodaTechnical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama, Japan.
Fang HeTechnical Research Laboratory, Takanashi Milk Products Co., Ltd., Yokohama, Japan.
Hideki TakahashiLaboratory of Plant Pathology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Julio VillenaFood and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Haruki KitazawaFood and Feed Immunology Group, Laboratory of Animal Products Chemistry, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Tohoku University · JPMegmilk Snow Brand (Japan) · JPBangladesh Agricultural University · BDChattogram Veterinary and Animal Sciences University · BDConsejo Nacional de Investigaciones Científicas y Técnicas · AR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adipocytes are the most important cell type in adipose tissue playing key roles in immunometabolism. We previously reported that nine members of the Toll-like receptor (TLR) family are expressed in an originally established porcine intramuscular pre-adipocyte (PPI) cell line. However, the ability of TLR ligands to modulate immunometabolic transcriptome modifications in porcine adipocytes has not been elucidated. Herein, we characterized the global transcriptome modifications in porcine intramuscular mature adipocytes (pMA), differentiated from PPI, following stimulation with Pam3csk4, Poly(I:C) or LPS which are ligands for TLR2, TLR3, and TLR4, respectively. Analysis of microarray data identified 530 (218 up, 312 down), 520 (245 up, 275 down), and 525 (239 up, 286 down) differentially expressed genes (DEGs) in pMA following the stimulation with Pam3csk4, Poly(I:C), and LPS, respectively. Gene ontology classification revealed that DEGs are involved in several biological processes including those belonging to immune response and lipid metabolism pathways. Functionally annotated genes were organized into two groups for downstream analysis: immune response related genes (cytokines, chemokines, complement factors, adhesion molecules, and signal transduction), and genes involved with metabolic and endocrine functions (hormones and receptors, growth factors, and lipid biosynthesis). Differential expression analysis revealed that

Indexed as

AdipocytesAdipogenesisAdipose TissueAnimalsCell LineFemaleGene Expression RegulationGene OntologyLipopeptidesLipopolysaccharidesMetabolic Networks and PathwaysMuscle, SkeletalOligonucleotide Array Sequence AnalysisPoly I-CRNA, MessengerSignal TransductionLipopeptidesLipopolysaccharidesPam(3)CSK(4) peptidePoly I-CRNA, MessengerToll-Like Receptor 2Toll-Like Receptor 3Toll-Like Receptor 4adipocytesimmunometabolismmicroarraypigTLRs (Toll-like receptors)transcriptome

Identifiers

PMID31191544
PMCPMC6549529
OpenAlexW2947193804

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.