ArticleFrontiers in microbiology2019
Improved GFP Variants to Study Gene Expression in Haloarchaea.
Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Construction of aACS omega · 2025Article
- Green Fluorescent Protein SELEX: Immobilization Chemistry and His-Tag Epitope Bias.Angewandte Chemie (International ed. in English) · 2025Article
- A colorful new reporter system to evaluate gene expression in the archaeon Haloferax volcanii.Extremophiles : life under extreme conditions · 2025Article
- Review
- Haloarchaea as Promising Chassis to Green Chemistry.Microorganisms · 2024Review
- 5'-untranslated region sequences enhance plasmid-based protein production inFrontiers in microbiology · 2024Article
- Recent Advances in the Study of Gas Vesicle Proteins and Application of Gas Vesicles in Biomedical Research.Life (Basel, Switzerland) · 2022Review
- Agrobacterium-mediated cassava transformation for the Asian elite variety KU50.Plant molecular biology · 2022Article
- Interaction of the gas vesicle proteins GvpA, GvpC, GvpN, and GvpO ofFrontiers in microbiology · 2022Article
- Reporter gene comparison demonstrates interference of complex body fluids with secreted luciferase activity.Scientific reports · 2021Article
- Levulinic Acid-Inducible and Tunable Gene Expression System forFrontiers in bioengineering and biotechnology · 2021Article
- A Synthetic Riboswitch to Regulate Haloarchaeal Gene Expression.Frontiers in microbiology · 2021Article
- Effect of Mutations in GvpJ and GvpM on Gas Vesicle Formation ofFrontiers in microbiology · 2021Article
- Review
- A bioluminescent reporter for the halophilic archaeon Haloferax volcanii.Extremophiles : life under extreme conditions · 2020Article
- Accessory Gvp Proteins Form a Complex During Gas Vesicle Formation of Haloarchaea.Frontiers in microbiology · 2020Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The study of promoter activities in haloarchaea is carried out exclusively using enzymes as reporters. An alternative reporter is the gene encoding the Green Fluorescent Protein (GFP), a simple and fast tool for investigating promoter strengths. However, the GFP variant smRS-GFP, used to analyze protein stabilities in haloarchaea, is not suitable to quantify weak promoter activities, since the fluorescence signal is too low. We enhanced the fluorescence of smRS-GFP 3.3-fold by introducing ten amino acid substitutions, resulting in mGFP6. Using mGFP6 as reporter, we studied six haloarchaeal promoters exhibiting different promoter strengths. The strongest activity was observed with the housekeeping promoters
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