Evidence map›Paper›PMID 31190160›Full record

ArticleJournal of molecular histology2019

Akt plays indispensable roles during the first cell lineage differentiation of mouse.

Songhua Xu, Lili Pang, Yue Liu, Xiuli Lian, Kaien Mo, Ruimin Lv, Huimin Zhu, Chengyu Lv, Jianmin Lin, Jiandong Sun and 2 more

Abstract read
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In one paragraph

Article in Journal of molecular histology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Songhua XuKey Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fuzhou, 350122, People's Republic of China.
Lili PangKey Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fuzhou, 350122, People's Republic of China.
Yue LiuKey Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fuzhou, 350122, People's Republic of China.
Xiuli LianDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
Kaien MoDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
Ruimin LvDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
Huimin ZhuFujian Key Laboratory of Medical Bioinformatics, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
Chengyu LvDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
Jianmin LinDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
Jiandong SunDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
Lixuan XuDepartment of Human Anatomy, Histology and Embryology, School of Basic Medical Sciences, Fujian Medical University, Fuzhou, 350122, People's Republic of China.
Shie WangKey Laboratory of Stem Cell Engineering and Regenerative Medicine of Fujian Province University, Fuzhou, 350122, People's Republic of China. shiewang@fjmu.edu.cn.ORCID http://orcid.org/0000-0001-6399-7037

Funding

SUBSTRATE SPECIFICITY OF GENETICALLY ALTERED PP60SRCF32CA009114 · NCI · UNIVERSITY OF CALIFORNIA BERKELEY · PI WEISS, BARBARA A · 1991 to 1992
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Fujian Provincial Health and Family Planning Research Talents Training Project 2017-1-67Fujian Provincial Project of Education and Science for Young and Middle-aged Teachers JAT170212Fujian Provincial Science and Technology Innovation Joint Fund Project 2017Y9114National Natural Science Foundation of China 81671526Science Fund for Distinguished Young Scholars of Fujian Province University 2018B028
6 · The paper itself

Abstract

The first cell lineage differentiation occurs during the development of mouse 8-cell embryo to blastocyst. Akt is a potent kinase whose role during blastocyst formation has not been elucidated. In the present study, immunofluorescence results showed that the Akt protein was specifically localized to the outer cells of the morula. Akt-specific inhibitor MK2206 significantly inhibited mouse blastocyst formation and resulted in decreased expression of the trophectoderm marker Cdx2 and led to granular distribution of ERα in the cytoplasm. Furthermore, knockdown of ERα by siRNA microinjection can also lead to a decrease in the development rate of mouse blastocysts, accompanied by a decrease in the expression level of Yap protein. We conclude that Akt may be indispensable for the first cell lineage differentiation of mouse.

Indexed as

Cell DifferentiationCell LineageAdaptor Proteins, Signal TransducingAnimalsBlastocystCell Cycle ProteinsEmbryo, MammalianEmbryonic DevelopmentGene Expression Regulation, DevelopmentalMiceMorulaProto-Oncogene Proteins c-aktYAP-Signaling ProteinsAdaptor Proteins, Signal TransducingCell Cycle ProteinsProto-Oncogene Proteins c-aktYap1 protein, mouseYAP-Signaling ProteinsAktBlastocyst formationImmunofluorescenceMouseYap

Identifiers

PMID31190160

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.