Evidence map›Paper›PMID 31190084›Full record

ArticleCellular and molecular life sciences : CMLS2019

Open reading frame mining identifies a TLR4 binding domain in the primary sequence of ECRG4.

Xitong Dang, Raul Coimbra, Liang Mao, Sonia Podvin, Xue Li, Hua Yu, Todd W Costantini, Xiaorong Zeng, Dana Larocca, Brian P Eliceiri and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular life sciences : CMLS, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Xitong DangDepartment of Surgery, University of California San Diego, San Diego, CA, 92103, USA.
Raul CoimbraDepartment of Surgery, University of California San Diego, San Diego, CA, 92103, USA.
Liang MaoThe Key Laboratory of Medical Electrophysiology, Ministry of Education, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, 646000, China.
Sonia PodvinDepartment of Surgery, University of California San Diego, San Diego, CA, 92103, USA.
Xue LiThe Key Laboratory of Medical Electrophysiology, Ministry of Education, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, 646000, China.
Hua YuThe Key Laboratory of Medical Electrophysiology, Ministry of Education, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, 646000, China.
Todd W CostantiniDepartment of Surgery, University of California San Diego, San Diego, CA, 92103, USA.
Xiaorong ZengThe Key Laboratory of Medical Electrophysiology, Ministry of Education, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, 646000, China.
Dana LaroccaMandala Biosciences, San Diego, CA, USA.
Brian P EliceiriDepartment of Surgery, University of California San Diego, San Diego, CA, 92103, USA.
Andrew BairdDepartment of Surgery, University of California San Diego, San Diego, CA, 92103, USA. abaird@ucsd.edu.ORCID http://orcid.org/0000-0003-0027-9905
Southwest Medical University · CNUniversity of California San Diego · USMandala Biosciences (United States) · US

Funding

Role of Src family kinases in endothelial cell biologyR01HL073396 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI ELICEIRI, BRIAN P · 2002 to 2011
$3.5M
A Combinatorial Approach to Wound Healing for Protein, Gene and Cell TherapeuticsP20GM078421 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BAIRD, ANDREW · 2006 to 2009
$3.4M
The Human-Specific Gene CHRFAM7A in LeukocytesR01GM121530 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI COSTANTINI, TODD W · 2017 to 2021
$1.6M
Targeting the choroid plexus for drug translocation into CSFR21EY018479 · NEI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI BAIRD, ANDREW · 2007 to 2010
$606k
National Natural Science Foundation of China 30-870-903National Natural Science Foundation of China (CN) 81-770-336NEI NIH HHS EY018479NEI NIH HHS R21 EY018479NHLBI NIH HHS HL73396NHLBI NIH HHS R01 HL073396NIGMS NIH HHS GM121530NIGMS NIH HHS P20 GM078421NIGMS NIH HHS P20-GM078421NIGMS NIH HHS R01 GM121530
6 · The paper itself

Abstract

The embedding of small peptide ligands within large inactive pre-pro-precursor proteins encoded by orphan open reading frames (ORFs) makes them difficult to identify and study. To address this problem, we generated oligonucleotide (< 100-400 base pair) combinatorial libraries from either the epidermal growth factor (EGF) ORF that encodes the > 1200 amino acid EGF precursor protein or the orphan ECRG4 ORF, that encodes a 148 amino acid Esophageal Cancer Related Gene 4 (ECRG4), a putative cytokine precursor protein of up to eight ligands. After phage display and 3-4 rounds of biopanning for phage internalization into prostate cancer epithelial cells, sequencing identified the 53-amino acid EGF ligand encoded by the 5' region of the EGF ORF and three distinct domains within the primary sequence of ECRG4: its membrane targeting hydrophobic signal peptide, an unanticipated amino terminus domain at ECRG4

Indexed as

Cell Line, TumorCell MovementCell Surface Display TechniquesGenes, Tumor SuppressorHumansHydrophobic and Hydrophilic InteractionsImmunity, InnateLigandsMaleNF-kappa BOligonucleotidesOpen Reading FramesProstatic NeoplasmsProtein DomainsToll-Like Receptor 4TransfectionECRG4 protein, humanLigandsNF-kappa BOligonucleotidesTLR4 protein, humanToll-Like Receptor 4Tumor Suppressor ProteinsCytokine precursorLigandPeptide targetingPhage displayReceptorSecretomeTumor suppressor gene

Identifiers

PMID31190084
PMCPMC11105628
OpenAlexW2952155844

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.