ArticlePlant signaling & behavior2019
An Arabidopsis protoplast isolation method reduces cytosolic acidification and activation of the chloroplast stress sensor SENSITIVE TO FREEZING 2.
Article in Plant signaling & behavior, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Remodeling of leaf lipidome during cold acclimation, de-acclimation, and re-acclimation influences frost tolerance of Festuca arundinacea.Plant & cell physiology · 2026Article
- Pan-Genomic Dissection of GH1 β-Glucosidases inPlants (Basel, Switzerland) · 2026Article
- SENSITIVE TO FREEZING2 is crucial for growth of Marchantia polymorpha under acidic conditions.Journal of plant research · 2024Article
- Sea-ATI unravels novel vocabularies of plant active cistrome.Nucleic acids research · 2023Article
- Oligogalactolipid production during cold challenge is conserved in early diverging lineages.Journal of experimental botany · 2023Article
- Phylogenetic and expression analyses of HSF gene families in wheat (Frontiers in plant science · 2022Article
- Biological Phase Separation and Biomolecular Condensates in Plants.Annual review of plant biology · 2021Review
- From Single Cell to Plants: Mesophyll Protoplasts as a Versatile System for Investigating Plant Cell Reprogramming.International journal of molecular sciences · 2020Review
- A Versatile High Throughput Screening Platform for Plant Metabolic Engineering Highlights the Major Role ofFrontiers in plant science · 2020Article
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Chloroplasts adapt to freezing and other abiotic stresses in part by modifying their membranes. One key-remodeling enzyme is SENSITIVE TO FREEZING2 (SFR2). SFR2 is unusual because it does not respond to initial cold stress or cold acclimation, instead it responds during freezing conditions in Arabidopsis. This response has been shown to be sensitive to cytosolic acidification. The unique lipid products of SFR2 have also been detected in response to non-freezing stresses, but what causes SFR2 to respond in these stresses is unknown. Here, we investigate protoplast isolation as a representative of wounding stress. We show that SFR2 oligogalactolipid products accumulate during protoplast isolation. Notably, we show that protoplast cytosol is acidified during isolation. Modification of the buffers reduces oligogalactolipid accumulation, while prolonged incubation in the isolated state increases it. We conclude that SFR2 activation during protoplast isolation correlates with cytosolic acidification, implying that all SFR2 activation may be dependent on cytosolic acidification. We also conclude that protoplasts can be more gently isolated, reducing their stress.
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