Evidence map›Paper›PMID 31187330›Full record

ArticleJournal of assisted reproduction and genetics2019

Cumulus cell pappalysin-1, luteinizing hormone/choriogonadotropin receptor, amphiregulin and hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 mRNA levels associate with oocyte developmental competence and embryo outcomes.

Richard J Kordus, Akhtar Hossain, Michael C Corso, Hrishikesh Chakraborty, Gail F Whitman-Elia, Holly A LaVoie

Abstract read
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In one paragraph

Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 2 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 2 syntheses or guidelines pooled it.

  1. Pooled it
  2. Pooled it
  3. Oocyte-cumulus cell interaction: a key factor in early embryo development.Biological reviews of the Cambridge Philosophical Society · 2026
    Review
  4. PAPPA's role in female reproduction.Reproduction (Cambridge, England) · 2025
    Review
  5. Review
  6. Article
  7. Review
  8. Article
  9. Oocyte Competence Biomarkers Associated With Oocyte Maturation: A Review.Frontiers in cell and developmental biology · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Richard J KordusDepartment of Cell Biology and Anatomy, University of South Carolina School of Medicine, Columbia, SC, USA.
Akhtar HossainDepartment of Epidemiology and Biostatistics, University of South Carolina, Columbia, SC, USA.
Michael C CorsoDepartment of Cell Biology and Anatomy, University of South Carolina School of Medicine, Columbia, SC, USA.
Hrishikesh ChakrabortyDuke Clinical Research Institute, Duke University, Durham, NC, USA.
Gail F Whitman-EliaAdvanced Fertility and Reproductive Endocrinology Institute, LLC, Columbia, SC, USA.
Holly A LaVoieDepartment of Cell Biology and Anatomy, University of South Carolina School of Medicine, Columbia, SC, USA. holly.lavoie@uscmed.sc.edu.ORCID http://orcid.org/0000-0002-6658-549X

Funding

School of Medicine, University of South Carolina RPMSUniversity of South Carolina ASPIRE-I
6 · The paper itself

Abstract

purposeTo determine whether a selected set of mRNA biomarkers expressed in individual cumulus granulosa cell (CC) masses show association with oocyte developmental competence, embryo ploidy status, and embryo outcomes.

methodsThis prospective observational cohort pilot study assessed levels of mRNA biomarkers in 163 individual CC samples from 15 women stimulated in antagonist cycles. Nineteen mRNA biomarker levels were measured by real-time PCR and related to the development of their corresponding individually cultured oocytes and subsequent embryos, embryo ploidy status, and live birth outcomes.

resultsPAPPA mRNA levels were significantly higher in CC from oocytes that led to euploid embryos resulting in live births and aneuploid embryos compared to immature oocytes by ANOVA. LHCGR mRNA levels were significantly higher in CC of oocytes resulting in embryos associated with live birth compared to immature oocytes and oocytes resulting in arrested embryos by ANOVA. Using a general linearized mixed model to assess ploidy status, CC HSD3B mRNA levels in oocytes producing euploid embryos were significantly lower than other oocyte outcomes, collectively. When transferred euploid embryos outcomes were analyzed by ANOVA, AREG mRNA levels were significantly lower and PAPPA mRNA levels significantly higher in CC from oocytes that produced live births compared to transferred embryos that did not form a pregnancy.

conclusionsCollectively, PAPPA, LHCGR, and AREG mRNA levels in CC may be able to identify oocytes with the best odds of resulting in a live birth, and HSD3B1 mRNA levels may be able to identify oocytes capable of producing euploid embryos.

Indexed as

AdultAmphiregulinCumulus CellsEmbryonic DevelopmentEmbryo TransferFemaleGene Expression Regulation, DevelopmentalHumansIn Vitro Oocyte Maturation TechniquesMultienzyme ComplexesOocyte RetrievalOocytesOogenesisOvarian FolliclePloidiesPregnancy3 beta-hydroxysteroid oxidoreductase-delta(5) 3-ketosteroid isomeraseAmphiregulinAREG protein, humanLHCGR protein, humanMultienzyme ComplexesPAPPA protein, humanPregnancy-Associated Plasma Protein-AProgesterone ReductaseReceptors, LHRNA, MessengerSteroid IsomerasesCumulus cellsEuploid embryomRNA levelsOocyte developmental competenceReal-time PCR

Identifiers

PMID31187330
PMCPMC6657401

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.