ArticleCell transplantation
The Efficacy of a Scaffold-free Bio 3D Conduit Developed from Autologous Dermal Fibroblasts on Peripheral Nerve Regeneration in a Canine Ulnar Nerve Injury Model: A Preclinical Proof-of-Concept Study.
Article in Cell transplantation. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Animal models in peripheral nerve transection studies: a systematic review on study design and outcomes assessment.Regenerative medicine · 2024Pooled it
- Challenges and opportunities in generating microvasculature using bioprinting techniques.npj biomedical innovations · 2026Review
- Advances in 3D printing combined with tissue engineering for nerve regeneration and repair.Journal of nanobiotechnology · 2025Review
- Facial Nerve Regeneration in Immunodeficient Rats Using a Bio 3D Conduit Fabricated From Human Dental Pulp Stem Cells.Stem cells international · 2025Article
- Spherical Shell Bioprinting to Produce Uniform Spheroids with Controlled Sizes.Journal of functional biomaterials · 2024Article
- Functional Assessment of Rat Sciatic Nerve Regeneration Using a Nerve Conduit Based on Ion Exchange Membrane.Bulletin of experimental biology and medicine · 2024Article
- Fabrication of Artificial Nerve Conduits Used in a Long Nerve Gap: Current Reviews and Future Studies.Bioengineering (Basel, Switzerland) · 2024Review
- Peripheral nerve regeneration following scaffold-free conduit transplant of autologous dermal fibroblasts: a non-randomised safety and feasibility trial.Communications medicine · 2024Article
- Article
- Potential Application of Orofacial MSCs in Tissue Engineering Nerve Guidance for Peripheral Nerve Injury Repair.Stem cell reviews and reports · 2023Review
- Regenerative Strategies in Treatment of Peripheral Nerve Injuries in Different Animal Models.Tissue engineering and regenerative medicine · 2023Review
- Horizon Scanning in Tissue Engineering Using Citation Network Analysis.Therapeutic innovation & regulatory science · 2023Article
- Mesenchymal Stem Cells in Nerve Tissue Engineering: Bridging Nerve Gap Injuries in Large Animals.International journal of molecular sciences · 2023Review
- Advances in nerve guidance conduits for peripheral nerve repair and regeneration.American journal of stem cells · 2023Review
- Nerve regeneration using the Bio 3D nerve conduit fabricated with spheroids.Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs · 2022Review
- 3D Printed Personalized Nerve Guide Conduits for Precision Repair of Peripheral Nerve Defects.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Review
- ID1/ID3 mediate the contribution of skin fibroblasts to local nerve regeneration through Itga6 in wound repair.Stem cells translational medicine · 2021Article
- Pilot study on the effects of low intensity focused ultrasound in a swine model of neuropathic pain.Journal of neurosurgery · 2021Article
- Development of a common peroneal nerve injury model in domestic swine for the study of translational neuropathic pain treatments.Journal of neurosurgery · 2021Article
- A Review of Recent Advances in 3D Bioprinting With an Eye on Future Regenerative Therapies in Veterinary Medicine.Frontiers in veterinary science · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autologous nerve grafting is widely accepted as the gold standard treatment for segmental nerve defects. To overcome the inevitable disadvantages of the original method, alternative methods such as the tubulization technique have been developed. Several studies have investigated the characteristics of an ideal nerve conduit in terms of supportive cells, scaffolds, growth factors, and vascularity. Previously, we confirmed that biological scaffold-free conduits fabricated from human dermal fibroblasts promote nerve regeneration in a rat sciatic nerve injury model. The purpose of this study is to evaluate the feasibility of biological scaffold-free conduits composed of autologous dermal fibroblasts using a large-animal model. Six male beagle dogs were used in this study. Eight weeks before surgery, dermal fibroblasts were harvested from their groin skin and grown in culture. Bio 3D conduits were assembled from proliferating dermal fibroblasts using a Bio 3D printer. The ulnar nerve in each dog's forelimb was exposed under general anesthesia and sharply cut to create a 5 mm interstump gap, which was bridged by the prepared 8 mm Bio 3D conduit. Ten weeks after surgery, nerve regeneration was investigated. Electrophysiological studies detected compound muscle action potentials (CMAPs) of the hypothenar muscles and motor nerve conduction velocity (MNCV) in all animals. Macroscopic observation showed regenerated ulnar nerves. Low-level hypothenar muscle atrophy was confirmed. Immunohistochemical, histological, and morphometric studies confirmed the existence of many myelinated axons through the Bio 3D conduit. No severe adverse event was reported. Hypothenar muscles were re-innervated by regenerated nerve fibers through the Bio 3D conduit. The scaffold-free Bio 3D conduit fabricated from autologous dermal fibroblasts is effective for nerve regeneration in a canine ulnar nerve injury model. This technology was feasible as a treatment for peripheral nerve injury and segmental nerve defects in a preclinical setting.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.