ArticleProtoplasma2019
Technique for the identification of osmophores in flowers of herbarium material (TIOFH).
Article in Protoplasma, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Chemical, morphological, and genetic characterization of the floral scent and scent-releasing structures of Gynandropsis gynandra (Cleomaceae, Brassicales).Plant biology (Stuttgart, Germany) · 2025Article
- Anatomy and ultrastructure floral osmophores of Catasetum fimbriatum (Orchidaceae).Protoplasma · 2021Article
- Do food trichomes occur in Pinguicula (Lentibulariaceae) flowers?Annals of botany · 2020Article
- Localization, morphology, anatomy and ultrastructure of osmophores in species of Rhamnaceae.Protoplasma · 2020Article
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Authors and funding
2 authors.
Funding
Abstract
The histochemical studies that search for osmophores differ in the protocol they follow for fixation and discoloration of the samples, and also in the type and number of stains utilized. Despite these differences, all the studies have one point in common: the use of fresh material either collected directly in the field or cultivated in botanical gardens and greenhouses. This is an obvious limitation for the studies of osmophores. Flower parts of herbarium specimens of different dates of collection and different plant families were exposed to variable times and percentages of discoloration agents and under different stains for finding if it is possible to positively test osmophores in this type of material. We obtained positive results discoloring the samples with ethyl alcohol 96° and sodium hypochlorite, and staining with Lugol, Oil Red O, and Neutral Red (TIOFH). A protocol (TIOFH3) for osmophore testing that combines these three stains into a single procedure is proposed.
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