Evidence map›Paper›PMID 31183428›Full record

ArticleHeliyon2019

Angiogenic properties of dental pulp stem cells conditioned medium on endothelial cells

Sueli Patricia Harumi Miyagi de Cara, Clarice Silvia Taemi Origassa, Fernando de Sá Silva, Maria Stella N A Moreira, Danilo Candido de Almeida, Ana Clara Fagundes Pedroni, Giovanna Lopes Carvalho, Diego Pulzatto Cury, Niels Olsen Saraiva Câmara, Márcia Martins Marques

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 1 synthesis or guideline pooled it.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sueli Patricia Harumi Miyagi de CaraSchool of Dentistry, Centro Universitário das Faculdades Metropolitanas Unidas (FMU), São Paulo, SP, Brazil.
Clarice Silvia Taemi OrigassaDepartamento de Medicina, Divisão de Nefrologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Fernando de Sá SilvaInstitute of Life Sciences, Universidade Federal de Juiz de Fora (UFJF), Governador Valadares, MG, Brazil.
Maria Stella N A MoreiraSchool of Dentistry, Universidade Ibirapuera (UNIB), Sao Paulo, SP, Brazil.
Danilo Candido de AlmeidaDepartamento de Medicina, Divisão de Nefrologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Ana Clara Fagundes PedroniDepartment of Restorative Dentistry and Endodontics, School of Dentistry, University of Sao Paulo (USP), Sao Paulo, SP, Brazil.
Giovanna Lopes CarvalhoDepartment of Restorative Dentistry and Endodontics, School of Dentistry, University of Sao Paulo (USP), Sao Paulo, SP, Brazil.
Diego Pulzatto CurySchool of Medicine, UNINOVE University, São Paulo, São Paulo, Brazil.
Niels Olsen Saraiva CâmaraDepartamento de Medicina, Divisão de Nefrologia, Universidade Federal de São Paulo, São Paulo, SP, Brazil.
Márcia Martins MarquesDepartment of Restorative Dentistry and Endodontics, School of Dentistry, University of Sao Paulo (USP), Sao Paulo, SP, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTo evaluate the effect of SHED-CM on the proliferation, differentiation, migration ability, cell death, gene expression and production of VEGF of HUVEC

methodsThree culture media [M199, DMEM/Ham's F12 and DMEM/Ham's F12 conditioned by SHEDs] were used as experimental groups. SHED-CM was prepared maintaining confluent cells in culture without serum for 3 days. The proliferation and cell death marker of HUVECs were assessed using flow cytometry. The capacity of formation of vascular-like structures was analyzed in cells grown over Matrigel

resultsSHED-CM promoted significantly lower expression of 7AAD in HUVECs; whereas the expression of the Ki67 was similar in all groups. The vascular-like structures were observed in all groups. Migration of SHED-CM group was faster than DMEM/Ham's F12. SHED-CM induced similar expression of VEGF-A than M199, and higher than DMEM/Ham's F12. SHED-CM induced significantly higher VEGF synthesis than other media. SHED-CM induced formation of a vascularized connective tissue inside the root canal.

conclusionThe study showed that SHEDs release angiogenic and cytoprotective factors, which are of great importance for tissue engineering. CLINICAL SIGNIFICANCE: SHED-CM could be an option to the use of stem cells in tissue engineering.

Indexed as

BioengineeringCell biology

Identifiers

PMID31183428
PMCPMC6488540

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.