ArticleJournal of cell science2019
Rho kinase-dependent apical constriction counteracts M-phase apical expansion to enable mouse neural tube closure.
Article in Journal of cell science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Mechanosensitive feedback organizes cell shape and motion during hindbrain neuropore morphogenesis.Current biology : CB · 2026Article
- An active matter model captures spatial dynamics of actomyosin oscillations in larval epithelial cells duringProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Discovering the unexpected: Insights into the dynamics of mouse neural tube closure revealed by time-lapse imaging.Developmental biology · 2026Review
- The Mechanics of Building Functional Organs.Cold Spring Harbor perspectives in biology · 2025Review
- Quantifying mechanical forces during vertebrate morphogenesis.Nature materials · 2024Article
- Optical coherence tomography-guided Brillouin microscopy highlights regional tissue stiffness differences during anterior neural tube closure in the Mthfd1l murine mutant.Development (Cambridge, England) · 2024Article
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- The surface ectoderm exhibits spatially heterogenous tension that correlates with YAP localisation during spinal neural tube closure in mouse embryos.Cells & development · 2023Article
- A Shared Pathogenic Mechanism for Valproic Acid and SHROOM3 Knockout in a Brain Organoid Model of Neural Tube Defects.bioRxiv : the preprint server for biology · 2023Article
- The cellular dynamics of neural tube formation.Biochemical Society transactions · 2023Article
- Synchronisation of apical constriction and cell cycle progression is a conserved behaviour of pseudostratified neuroepithelia informed by their tissue geometry.Developmental biology · 2023Article
- Cell cycle dynamics control fluidity of the developing mouse neuroepithelium.Nature physics · 2023Article
- Two-Photon Cell and Tissue Level Laser Ablation Methods to Study Morphogenetic Biomechanics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Scribble mutation disrupts convergent extension and apical constriction during mammalian neural tube closure.Developmental biology · 2021Article
- Hindbrain neuropore tissue geometry determines asymmetric cell-mediated closure dynamics in mouse embryos.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Intrinsic Mechanical Cues and Their Impact on Stem Cells and Embryogenesis.Frontiers in cell and developmental biology · 2021Review
- Refinement of inducible gene deletion in embryos of pregnant mice.Birth defects research · 2020Article
- Pulsed actomyosin contractions in morphogenesis.F1000Research · 2020Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Cellular generation of mechanical forces required to close the presumptive spinal neural tube, the 'posterior neuropore' (PNP), involves interkinetic nuclear migration (INM) and apical constriction. Both processes change the apical surface area of neuroepithelial cells, but how they are biomechanically integrated is unknown. Rho kinase (Rock; herein referring to both ROCK1 and ROCK2) inhibition in mouse whole embryo culture progressively widens the PNP. PNP widening is not caused by increased mechanical tension opposing closure, as evidenced by diminished recoil following laser ablation. Rather, Rock inhibition diminishes neuroepithelial apical constriction, producing increased apical areas in neuroepithelial cells despite diminished tension. Neuroepithelial apices are also dynamically related to INM progression, with the smallest dimensions achieved in cells positive for the pan-M phase marker Rb phosphorylated at S780 (pRB-S780). A brief (2 h) Rock inhibition selectively increases the apical area of pRB-S780-positive cells, but not pre-anaphase cells positive for phosphorylated histone 3 (pHH3
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Registered trials
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