ArticleBMC infectious diseases2019
Use of nested PCR for the detection of trichomonads in bronchoalveolar lavage fluid.
Article in BMC infectious diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Detection of trichomonads in induced sputum from asthma patients in Korea.Parasites, hosts and diseases · 2025Article
- Prevalence and genetic diversity of Pentatrichomonas hominis in pig populations in Guangdong and Anhui Provinces, China.Parasite (Paris, France) · 2025Article
- Prospective observational study ofHeliyon · 2024Article
- Clinical implications of trichomonads detected in bronchoalveolar fluid by metagenomic next-generation sequencing: a multicenter retrospective study.Frontiers in cellular and infection microbiology · 2024Article
- Development of Nested Polymerase Chain Reaction with Novel Specific Primers for Detection ofAnimals : an open access journal from MDPI · 2023Article
- Article
- Pyopneumothorax Caused byInfection and drug resistance · 2022Article
- Sporadic isolation ofParasitology · 2021Article
- SARS-CoV-2 and superimposed infection by trichomonads.The Journal of infection · 2021Article
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Authors and funding
7 authors.
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Abstract
backgroundThe methods routinely used to detect trichomonads in the lungs are not sensitive enough, and an effective method is urgently needed.
methodPrimers were first designed to match the conserved area of the 18S rRNA gene of trichomonads. Then, nested PCR was carried out to detect trichomonads in bronchoalveolar lavage fluid (BALF). Finally, all positive specimens were subjected to DNA sequencing and phylogenetic analysis.
resultsAmong 115 bronchoalveolar lavage fluid samples, ten samples tested positive in nested PCR (10/115), while no samples were positive in wet mount microscopy (0/115) (P < 0.01). Among the ten positive specimens, two were identified as Tetratrichomonas spp. and the other eight as Trichomonas tenax in phylogenetic analysis.
conclusionsNested PCR is an effective way to detect trichomonads in bronchoalveolar lavage fluid.
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