ArticleBMC plant biology2019
Agrobacterium-mediated vacuum infiltration and floral dip transformation of rapid-cycling Brassica rapa.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- The HD-ZIP I transcription factor BcLMI1 regulates bolting time inHorticulture research · 2026Article
- A simple and reliable method for determining kanamycin sensitivity in Brassica napus.Plant physiology · 2026Article
- Genetically Modified Plants in Agriculture.Biology · 2026Review
- Overexpression of the glutathione synthase gene PsGSH2 enhances cadmium stress tolerance in transgenic Arabidopsis thaliana.Plant cell reports · 2025Article
- Review
- Plant genetic transformation: achievements, current status and future prospects.Plant biotechnology journal · 2025Review
- Non-tissue culture genetic modifications for plant improvement.Plant molecular biology · 2025Review
- Lost in translation: What we have learned from attributes that do not translate from Arabidopsis to other plants.The Plant cell · 2025Review
- StACS3-mediated drought stress adaptation in potato involves interactions with StPP2C2 and St14-3-3 proteins.Frontiers in plant science · 2025Article
- Review
- Establishment of a Genetic Transformation and Gene Editing Method by Floral Dipping inPlants (Basel, Switzerland) · 2024Article
- Agrobacterium-Mediated Transformation for the Development of Transgenic Crops; Present and Future Prospects.Molecular biotechnology · 2024Review
- Efficient floral dip transformation method usingSaudi journal of biological sciences · 2023Article
- Optimization of Regeneration andPlants (Basel, Switzerland) · 2022Article
- Genome-Wide Identification of ATG Gene Family Members in Fagopyrum tataricum and Their Expression during Stress ResponsesInternational journal of molecular sciences · 2022Article
- BcGR1.1, a Cytoplasmic Localized Glutathione Reductase, Enhanced Tolerance to Copper Stress inAntioxidants (Basel, Switzerland) · 2022Article
- A simple method forPlant biotechnology (Tokyo, Japan) · 2021Article
- Functional Characterization ofFrontiers in plant science · 2021Article
- Generation of Transgenic Rice Expressing CircRNA and Its Functional Characterization.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Ectopic expression of nucleolar DEAD-Box RNA helicase OsTOGR1 confers improved heat stress tolerance in transgenic Chinese cabbage.Plant cell reports · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundRapid-cycling Brassica rapa (RCBr), also known as Wisconsin Fast Plants, are small robust plants with a short lifecycle that are widely used in biology teaching. RCBr have been used for decades but there are no published reports of RCBr genetic transformation. Agrobacterium-mediated vacuum infiltration has been used to transform pakchoi (Brassica rapa ssp. chinensis) and may be suitable for RCBr transformation. The floral dip transformation method, an improved version of vacuum infiltration, could make the procedure easier.
resultsBased on previous findings from Arabidopsis and pakchoi, plants of three different ages were inoculated with Agrobacterium. Kanamycin selection was suboptimal with RCBr; a GFP screen was used to identify candidate transformants. RCBr floral bud dissection showed that only buds with a diameter less than 1 mm carried unsealed carpels, a key point of successful floral dip transformation. Plants across a wide range of inflorescence maturities but containing these immature buds were successfully transformed, at an overall rate of 0.1% (one per 1000 T
conclusionA genetic transformation system for RCBr was established in this study. This will promote development of new biology teaching tools as well as basic biology research on Brassica rapa.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.