ArticleClinical and experimental reproductive medicine2019
Does conventional freezing affect sperm DNA fragmentation?
Article in Clinical and experimental reproductive medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
28 citing papers in PubMed, 40 citations in OpenAlex.
- Combined effects of urine exposure and cryopreservation on sperm quality: an in vitro study of retrograde ejaculation.Asian journal of andrology · 2026Article
- Article
- The impact of fresh versus cryopreserved testicular sperm on in-vitro fertilization outcomes in couples with elevated sperm DNA fragmentation: a retrospective cohort study.Reproductive biology and endocrinology : RB&E · 2026Article
- Protective Effects of Curcumin on Cryopreserved Human Sperm Through Mitochondrial Apoptosis Regulation.Journal of family & reproductive health · 2025Article
- Optimization of Duck Semen Freezing Procedure and Regulation of Oxidative Stress.Animals : an open access journal from MDPI · 2025Article
- Hurdles of Sperm Success: Exploring the Role of DNases.International journal of molecular sciences · 2025Review
- Effects of astaxanthin supplementation during vitrification and liquid nitrogen vapor freezing on motility, morphology, survival, reactive oxygen species (ROS), and DNA fragmentation of post-cryopreserved human sperm.JBRA assisted reproduction · 2024Article
- The correlation between abnormal Krüger strict morphology and the sperm DNA fragmentation index.JBRA assisted reproduction · 2024Article
- Assessing the Impact of the Novel Sperm Selection Technique 'Annexin-V Coated Polystyrene Bead Technique' on Mouse Assisted Reproductive Techniques Outcomes: Preliminary Findings.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- The evaluation effect of nanoliposome-loaded Mito-Tempo on sperm parameters during human sperm cryopreservation.Journal of assisted reproduction and genetics · 2024Article
- Poor Sperm Chromatin Condensation Is Associated with Cryopreservation-Induced DNA Fragmentation and Cell Death in Human Spermatozoa.Journal of clinical medicine · 2024Article
- Sperm DNA Fragmentation: causes, evaluation and management in male infertility.JBRA assisted reproduction · 2024Review
- Impact of cryoprotectant-free sperm vitrification in pulled-glass capillary on sperm parameters and DNA integrity: A lab trial study.International journal of reproductive biomedicine · 2024Article
- Effect of freezing and thawing on ejaculated sperm and subsequent pregnancy and neonatal outcomes in IVF.Frontiers in endocrinology · 2024Article
- Controversy and Consensus on Indications for Sperm DNA Fragmentation Testing in Male Infertility: A Global Survey, Current Guidelines, and Expert Recommendations.The world journal of men's health · 2023Article
- Article
- Analysis of sperm separation protocols for isolating cryopreserved human spermatozoa.Reproduction & fertility · 2023Article
- Cryopreservation of Human Spermatozoa: Functional, Molecular and Clinical Aspects.International journal of molecular sciences · 2023Review
- Biomaterials for Testicular Bioengineering: How far have we come and where do we have to go?Frontiers in endocrinology · 2023Review
- Outcomes of Frozen Oocyte Donor In Vitro Fertilization (IVF) Cycles Using Fresh Versus Frozen Sperm.Reproductive sciences (Thousand Oaks, Calif.) · 2022Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveSperm cryopreservation has been widely used in assisted reproductive technology, as it offers great potential for the treatment of some types of male infertility. However, cryopreservation may result in changes in membrane lipid composition and acrosome status, as well as reductions in sperm motility and viability. This study aimed to evaluate sperm DNA fragmentation damage caused by conventional freezing using the sperm chromatin dispersion test.
methodsIn total, 120 fresh human semen samples were frozen by conventional methods, using SpermFreeze Solution as a cryoprotectant. Routine semen analysis and a Halosperm test (using the Halosperm kit) were performed on each sample before freezing and after thawing. Semen parameters and sperm DNA fragmentation were compared between these groups.
resultsThere was a significant decrease in sperm progressive motility, viability, and normal morphology after conventional freezing (32.78%, 79.58%, and 3.87% vs. 16%, 55.99%, and 2.55%, respectively). The sperm head, midpiece, and tail defect rate increased slightly after freezing. Furthermore, the DNA fragmentation index (DFI) was significantly higher after thawing than before freezing (19.21% prior to freezing vs. 22.23% after thawing). Significant increases in the DFI after cryopreservation were observed in samples with both normal and abnormal motility and morphology, as well as in those with normal viability.
conclusionConventional freezing seems to damage some sperm parameters, in particular causing a reduction in sperm DNA integrity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.