ArticleAnimals : an open access journal from MDPI2019
Developmental Competence of Domestic Cat Vitrified Oocytes in 3D Enriched Culture Conditions.
Article in Animals : an open access journal from MDPI, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Development of a three-dimensional vitrification protocol for domestic cat cumulus-oocyte complexes and comparison with standard vitrification.Frontiers in veterinary science · 2026Article
- Extracorporal Ovary Bioreactor System with Oxygen Supply and Drug Delivery Option.Bioengineering (Basel, Switzerland) · 2025Article
- New Strategies for Conservation of Gentile di Puglia Sheep Breed, an Autochthonous Capital of Millennial Tradition in Southern Italy.Animals : an open access journal from MDPI · 2023Article
- Fighting Like Cats and Dogs: Challenges in Domestic Carnivore Oocyte Development and Promises of Innovative Culture Systems.Animals : an open access journal from MDPI · 2021Review
- Article
- Comparison of Different Materials for Self-Pressurized Vitrification of Feline Oocytes-First Results.Animals : an open access journal from MDPI · 2021Article
- Follicular extracellular vesicles enhance meiotic resumption of domestic cat vitrified oocytes.Scientific reports · 2020Article
- Inhibition of Apoptotic Pathways Improves DNA Integrity but Not Developmental Competence of Domestic Cat Immature Vitrified Oocytes.Frontiers in veterinary science · 2020Article
- One-step automated bioprinting-based method for cumulus-oocyte complex microencapsulation for 3D in vitro maturation.PloS one · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cryoinjuries severely affect the competence of vitrified oocytes (VOs) to develop into embryos after warming. The use of culture conditions that provide physical and chemical support and resemble the in vivo microenvironment in which oocytes develop, such as 3D scaffolds and coculture systems, might be useful to improve VOs outcomes. In this study, an enriched culture system of 3D barium alginate microcapsules was employed for the in vitro embryo production of domestic cat VOs. Cryotop vitrified-warmed oocytes were in vitro matured for 24 h in the 3D system with or without fresh cumulus-oocyte complexes (COCs) in coculture, whereas a control group of VOs was cultured in traditional 2D microdrops of medium. After in vitro fertilization, presumptive embryos were cultured in 3D or 2D systems according to the maturation conditions. Vitrified oocytes were able to mature and develop into embryos in 3D microcapsules (17.42 ± 11.83%) as well as in 2D microdrops (14.96 ± 8.80%), but the coculture with companion COCs in 3D resulted in similar proportions of VOs embryo development (18.39 ± 16.67%;
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.