ArticleJournal of virological methods2019
Duplex real-time RT-PCR assay for detection and subgroup-specific identification of human respiratory syncytial virus.
Article in Journal of virological methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
- Results From the Third and Fourth WHO External Quality Assessments for the Molecular Detection of Respiratory Syncytial Virus.Influenza and other respiratory viruses · 2026Article
- From plant pathogen to public health tool: A review of tobamoviruses and their role in wastewater & environmental surveillance.Journal of water and health · 2026Review
- From Triplex to Quadruplex: Enhancing CDC's Respiratory qPCR Assay with RSV Detection on Panther FusionMicroorganisms · 2026Article
- Rapid, sensitive, and highly specific diagnosis of respiratory syncytial virus using recombinase polymerase amplification-based biosensor and fluorescence detection.Frontiers in cellular and infection microbiology · 2026Article
- Article
- Genetic and Epidemiological Insights Into Respiratory Syncytial Virus Infections: A Comparative Study of Hospitalized Versus Community Cases in Portugal (2021-2023).Influenza and other respiratory viruses · 2025Article
- Oral dosing of the nucleoside analog obeldesivir is efficacious against RSV infection in African green monkeys.Nature communications · 2025Article
- An Improved Rapid and Sensitive Long Amplicon Method for Nanopore-Based RSV Whole-Genome Sequencing.Influenza and other respiratory viruses · 2025Article
- Impact of COVID-19 on Respiratory Virus Infections in Children, Japan, 2018-2023.Immunity, inflammation and disease · 2025Article
- Epidemiology of respiratory viruses according to age group, 2023-24 winter season, Kyoto, Japan.Scientific reports · 2025Article
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- Simple SARS-CoV-2 concentration methods for wastewater surveillance in low resource settings.The Science of the total environment · 2024Article
- Evidence of the simultaneous replications of active viruses in specimens positive for multiple respiratory viruses.Microbiology spectrum · 2024Article
- Etiology of Acute Lower Respiratory Illness Hospitalizations Among Infants in 4 Countries.Open forum infectious diseases · 2023Article
- Genomic Sequencing Surveillance to Identify Respiratory Syncytial Virus Mutations, Arizona, USA.Emerging infectious diseases · 2023Article
- Molecular Diversity of Human Respiratory Syncytial Virus before and during the COVID-19 Pandemic in Two Neighboring Japanese Cities.Microbiology spectrum · 2023Article
- Respiratory Syncytial Virus Infection Among Hospitalized Infants in Four Middle-Income Countries.Journal of the Pediatric Infectious Diseases Society · 2023Article
- Reference material development for detection of human respiratory syncytial virus using digital PCR.Analytical and bioanalytical chemistry · 2023Article
- RAPIDViruses · 2023Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human respiratory syncytial virus (HRSV) is a leading cause of acute respiratory illness in young children worldwide. Reliable detection and identification of HRSV subgroup A and B infections are essential for accurate disease burden estimates in anticipation of licensure of novel HRSV vaccines and immunotherapies. To ensure continued reliability, molecular assays must remain current with evolving virus strains. We have developed a HRSV subgroup-specific real-time RT-PCR (rRT-PCR) assay for detection and subgroup identification using primers and subgroup-specific probes targeting a conserved region of the nucleoprotein gene combined in a single duplex reaction using all genome sequence data currently available in GenBank. The assay was validated for analytical sensitivity, specificity, reproducibility, and clinical performance with a geographically diverse collection of viral isolates and respiratory specimens in direct comparison with an established pan-HRSV rRT-PCR reference test. The assay was sensitive, reproducibly detecting as few as 5-10 copies/reaction of target RNA. The assay was specific, showing no amplification with a panel of 16 other common respiratory pathogens or predicted by in silico primer/probe analysis. The duplex rRT-PCR assay based on the most current available genome sequence data permits rapid, sensitive and specific detection and subgroup identification of HRSV.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.