miR‑148a modulates the viability, migration and invasion of oral squamous cell carcinoma cells by regulating HLA‑G expression.

Oral squamous cell carcinoma (OSCC) is a common malignancy of the oral and maxillofacial regions. MicroRNAs (miRs) are a group of endogenous small noncoding RNAs that inhibit gene expression by binding to the mRNA of target genes, and serve important roles in numerous biological processes. Reverse transcription‑quantitative polymerase chain reaction and western blotting were used to detect gene and protein expression levels, respectively. Cell proliferation, migration and invasion were detected using MTT, wound healing and Matrigel assays, respectively. The association between miR‑148a and human leukocyte antigen‑G (HLA‑G) was analyzed using Targetscan and Luciferase reporter assays. In the present study, miR‑148a was revealed to be significantly downregulated in OSCC cells. To further investigate the functions of miR‑148a in OSCC, the viability, migration, and invasive abilities of SCC‑9 cells were investigated following transfection with miR‑148a mimics or miR‑148a inhibitor. It was revealed that transfection with miR‑148a mimics significantly reduced the viability, migration and invasion of cells, whereas miR‑148a inhibitor significantly enhanced these properties. In addition, HLA‑G was identified as a direct target of miR‑148a and demonstrated to be downregulated in OSCC cells. Furthermore, it was revealed that transfection with miR‑148a mimics decreased the expression levels of HLA‑G mRNA and protein in SCC‑9 cells, whereas transfection with miR‑148a inhibitor increased the expression of HLA‑G mRNA and protein. The results indicated that there was an association between miR‑148a and HLA‑G expression, and suggested that miR‑148a may be a potential target in the treatment of OSCC.