ArticleeLife2019
Nanoresolution real-time 3D orbital tracking for studying mitochondrial trafficking in vertebrate axons in vivo.
Article in eLife, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Mapping Nanoscale Forces and Potentials in Live Cells with Microsecond 3D Single-Particle Tracking.Chemical & biomedical imaging · 2026Article
- Formulation Strategies for Immunomodulatory Natural Products in 3D Tumor Spheroids and Organoids: Current Challenges and Emerging Solutions.Pharmaceutics · 2025Review
- Information optimization of laser scanning microscopes for real-time feedback-driven single particle tracking.Optics express · 2023Article
- Combined online Bayesian and windowed estimation of background and signal localization facilitates active-feedback particle tracking in complex environments.The Journal of chemical physics · 2022Article
- Real-Time Feedback-Driven Single-Particle Tracking: A Survey and Perspective.Small (Weinheim an der Bergstrasse, Germany) · 2022Review
- Multiphoton single-molecule localization by sequential excitation with light minima.Light, science & applications · 2022Article
- A common framework for single-molecule localization using sequential structured illumination.Biophysical reports · 2022Article
- Optimizing mitochondrial maintenance in extended neuronal projections.PLoS computational biology · 2021Article
- Information-Efficient, Off-Center Sampling Results in Improved Precision in 3D Single-Particle Tracking Microscopy.Entropy (Basel, Switzerland) · 2021Article
- The Role of Mitochondria in Optic Atrophy With Autosomal Inheritance.Frontiers in neuroscience · 2021Review
- Studying molecular interactions in the intact organism: fluorescence correlation spectroscopy in the living zebrafish embryo.Histochemistry and cell biology · 2020Review
- Back to the Future: Genetically Encoded Fluorescent Proteins as Inert Tracers of the Intracellular Environment.International journal of molecular sciences · 2020Review
- Trajectory data of antero- and retrograde movement of mitochondria in living zebrafish larvae.Data in brief · 2020Article
- Live cell imaging of single RNA molecules with fluorogenic Mango II arrays.Nature communications · 2020Article
- Single-Virus Tracking: From Imaging Methodologies to Virological Applications.Chemical reviews · 2020Review
- Pleiotropic Mitochondria: The Influence of Mitochondria on Neuronal Development and Disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Review
- Syntaphilin-Mediated Docking of Mitochondria at the Growth Cone Is Dispensable for Axon ElongationeNeuroArticle
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
We present the development and in vivo application of a feedback-based tracking microscope to follow individual mitochondria in sensory neurons of zebrafish larvae with nanometer precision and millisecond temporal resolution. By combining various technical improvements, we tracked individual mitochondria with unprecedented spatiotemporal resolution over distances of >100 µm. Using these nanoscopic trajectory data, we discriminated five motional states: a fast and a slow directional motion state in both the anterograde and retrograde directions and a stationary state. The transition pattern revealed that, after a pause, mitochondria predominantly persist in the original direction of travel, while transient changes of direction often exhibited longer pauses. Moreover, mitochondria in the vicinity of a second, stationary mitochondria displayed an increased probability to pause. The capability of following and optically manipulating a single organelle with high spatiotemporal resolution in a living organism offers a new approach to elucidating their function in its complete physiological context.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.