ArticleBMJ open ophthalmology2019
Next-generation sequencing for the detection of microorganisms present in human donor corneal preservation medium.
Article in BMJ open ophthalmology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Contact lens-associated infectious keratitis in physicians: a retrospective observational study.International ophthalmology · 2026Observational
- Evaluating the Molecular Potential and Interpretability of DNA in Historical Spirit Collection Media.Molecular ecology resources · 2026Article
- Clinical performance of nanopore targeted sequencing for diagnosing endophthalmitis.BMC microbiology · 2025Article
- Alterations in ocular fungal microbiota in patients with fungal keratitis: a comparative study from coastal regions of Eastern China.BMC ophthalmology · 2025Article
- Tear metabolomics reveals novel potential biomarkers in epithelial herpes simplex keratitis.BMC ophthalmology · 2025Article
- Aerosolization ocular surface microorganisms accumulation effect during non-contact tonometer measurements.BMC ophthalmology · 2024Article
- Unveiling challenging corneal infections: a comprehensive etiological diagnosis through metagenomic next-generation sequencing (mNGS) of corneal tissue samples.International ophthalmology · 2024Article
- Validation of metagenomic next-generation sequencing of bronchoalveolar lavage fluid for diagnosis of suspected pulmonary infections in patients with systemic autoimmune rheumatic diseases receiving immunosuppressant therapy.Frontiers in medicine · 2023Article
- Comparison of Microbial Sampling Sites and Donor-Related Factors on Corneal Graft Contamination.Journal of clinical medicine · 2022Article
- Exploring the Healthy Eye Microbiota Niche in a Multicenter Study.International journal of molecular sciences · 2022Article
- A Review on Pathogens and Necessary Diagnostic Work for Bleb-Related Infections (BRIs).Diagnostics (Basel, Switzerland) · 2022Review
- Clinical application of metagenomic next-generation sequencing in tuberculosis diagnosis.Frontiers in cellular and infection microbiology · 2022Article
- Review
- Shotgun sequencing to determine corneal infection.American journal of ophthalmology case reports · 2020Article
- Metagenomics in ophthalmology: Hypothesis or real prospective?Biotechnology reports (Amsterdam, Netherlands) · 2019Review
- Metagenome techniques to reduce diagnostic delay in Acanthamoeba keratitis.Romanian journal of ophthalmologyArticle
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTo detect the presence of microorganisms in the storage media of human donor corneas using next-generation sequencing method.
methodsSeven samples from organ culture (OC) group (Cornea Max, Eurobio, Les Ulis, France) with one control (sterile media without any cornea) and seven samples from hypothermic storage group (Cornea Cold, Eurobio) with one control were used for this study. The corneas were placed in the respective storage media for 14 days before collecting the samples. Storage media (2 mL) from each sample were collected in RNAase-free tubes and shipped for ribosomal RNA sequencing of 16 S and 18 S. Simultaneously, another 1 mL of media sample was used for conventional diagnostic method (CDM) using Bactec instruments.
resultsIn both, OC and hypothermic storage and control samples, the most abundant genera were
conclusionMetagenomics provides full taxonomic profiling of the detected genomic material of the organisms and thus has the potential to deliver a much wider microbiological diagnostic approach than CDM. The costs and turn-around time need to be reduced, and; the detection of viable organisms would help this technology to be introduced into routine clinical practice.
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