ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2019
Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Identification of a myosin 1B-binding aptamer for fluorescence imaging and targeted therapy of esophageal squamous cell carcinoma.Materials today. Bio · 2026Article
- Proximity-activated DNA scanning encoded sequencing for massive access to membrane proteins nanoscale organization.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Inhibition of SARS-CoV-2 replication by a ssDNA aptamer targeting the nucleocapsid protein.Microbiology spectrum · 2024Article
- Cell Membrane-Anchored DNA Nanoinhibitor for Inhibition of Receptor Tyrosine Kinase Signaling Pathways via Steric Hindrance and Lysosome-Induced Protein Degradation.ACS pharmacology & translational science · 2024Article
- Aptamer-functionalized field-effect transistor biosensors for disease diagnosis and environmental monitoring.Exploration (Beijing, China) · 2023Review
- Transferrin receptor-mediated internalization and intracellular fate of conjugates of a DNA aptamer.Molecular therapy. Nucleic acids · 2022Article
- Ultrasensitive DNA-Biomacromolecule Sensor for the Detection Application of Clinical Cancer Samples.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Aptamers used for molecular imaging and theranostics - recent developments.Theranostics · 2022Review
- Advances in aptamers against Aβ and applications in Aβ detection and regulation for Alzheimer's disease.Theranostics · 2022Review
- Aptamer Blocking Strategy Inhibits SARS-CoV-2 Virus Infection.Angewandte Chemie (International ed. in English) · 2021Article
- Advances in Optical Aptasensors for Early Detection and Diagnosis of Various Cancer Types.Frontiers in oncology · 2021Review
- Aptameric Probe Specifically Binding Protein Heterodimer Rather Than Monomers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019Article
Corrections and comments
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Authors and funding
8 authors.
Funding
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Abstract
Dimerization of proteins occurs frequently and plays integral roles in biological processes. However, no single molecular probe is available for in situ detection of protein dimers on cells and tissues because of the difficulty of isolating complete protein dimers for probe preparation and screening, which has greatly hampered the biomedical study of protein dimers. Herein, a G-rich DNA aptamer (termed BG2) that only binds alkaline phosphatase (AP) heterodimers rather than monomers is reported. This aptamer is generated by the cell-SELEX (systematic evolution of ligands by exponential enrichment) technique and proves to fold into a duplex stabilized antiparallel G-quadruplex structure. Using BG2 as molecular probe, AP heterodimers are found to be expressed on several kinds of cancer cells. As an affinity ligand, BG2 could isolate AP heterodimers from cell lysate. BG2 is also demonstrated to be applicable for tumor imaging in mice xenografted with cells highly expressing AP heterodimers. AP isozymes are found in several tissues and blood throughout the body, but the function and tissue distribution of AP heterodimers are totally unknown; therefore, BG2 could serve as a molecular probe to uncover the mystery of AP heterodimers. The generation of aptameric probes by cell-SELEX will open up a new situation for the study of protein dimers.
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