Evidence map›Paper›PMID 31178553›Full record

ArticleThe Journal of reproduction and development2019

The effects of electroporation on viability and quality of in vivo-derived bovine blastocysts.

Fuminori Tanihara, Maki Hirata, Shigeki Morikawa, Nhien Thi Nguyen, Quynh Anh LE, Takayuki Hirano, Yoshiyuki Fukumi, Toshiaki Abe, Takeshige Otoi

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of reproduction and development, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 53% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Fuminori TaniharaLaboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima 779-3233, Japan.
Maki HirataLaboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima 779-3233, Japan.
Shigeki MorikawaTokushima Prefectural Livestock Research Institute, Tokushima 779-3233, Japan.
Nhien Thi NguyenLaboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima 779-3233, Japan.
Quynh Anh LELaboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima 779-3233, Japan.
Takayuki HiranoLaboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima 779-3233, Japan.
Yoshiyuki FukumiTokushima Prefectural Livestock Research Institute, Tokushima 779-3233, Japan.
Toshiaki AbeTokushima Prefectural Livestock Research Institute, Tokushima 779-3233, Japan.
Takeshige OtoiLaboratory of Animal Reproduction, Faculty of Bioscience and Bioindustry, Tokushima University, Tokushima 779-3233, Japan.
Tokushima University · JPTokushima Prefectural General Education Center · JPTokushima Prefectural Central Hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The introduction of exogenous molecules into embryos is required for analyses of molecular dynamics and specific gene functions during early embryonic development. Electroporation is an effective method to transport exogenous molecules into cells, but is rarely used in bovine embryos. First, we evaluated the viability of in vivo-derived bovine blastocysts after electroporation with fluorescein (FAM) labeled-oligonucleotides with varying pulse numbers (3, 5, 7, and 10), while keeping the pulse duration at 1 msec and the electric field of 20 V/mm. Next, we examined the effects of zona pellucida status on blastocyst quality after electroporation, by comparing the average diameter of blastocysts before and after electroporation using blastocysts with intact zona pellucida and hatching/hatched blastocysts. Electroporation successfully introduced exogenous molecules into in vivo-derived bovine blastocysts without loss of viability. Moreover, the status of the zona pellucida may be associated with the quality of blastocysts after electroporation.

Indexed as

ElectroporationAnimalsBlastocystCattleCell CountCell SurvivalEmbryo, MammalianEmbryonic DevelopmentFemaleFertilization in VitroFluoresceinOligonucleotidesZona PellucidaFluoresceinOligonucleotidesBlastocystBovineElectroporationIn vivoZona pellucida

Identifiers

PMID31178553
PMCPMC6815737
OpenAlexW2949004521

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.