ArticleThe Journal of cell biology1987
An investigation of microtubule organization and functions in living Drosophila embryos by injection of a fluorescently labeled antibody against tyrosinated alpha-tubulin.
Article in The Journal of cell biology, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 83 citations in OpenAlex.
- Fluorescent Single-Walled Carbon Nanotubes for Protein Detection.Sensors (Basel, Switzerland) · 2019Review
- Quantitative microscopy uncovers ploidy changes during mitosis in liveBiology open · 2017Article
- Characterization of sub-nuclear changes in Caenorhabditis elegans embryos exposed to brief, intermediate and long-term anoxia to analyze anoxia-induced cell cycle arrest.BMC cell biology · 2005Article
- Reassessing the role and dynamics of nonmuscle myosin II during furrow formation in early Drosophila embryos.Molecular biology of the cell · 2004Article
- Live analysis of free centrosomes in normal and aphidicolin-treated Drosophila embryos.The Journal of cell biology · 1996Article
- The 95F unconventional myosin is required for proper organization of the Drosophila syncytial blastoderm.The Journal of cell biology · 1995Article
- Drosophila cytoplasmic dynein, a microtubule motor that is asymmetrically localized in the oocyte.The Journal of cell biology · 1994Article
- Maternal effect mutations of the sponge locus affect actin cytoskeletal rearrangements in Drosophila melanogaster embryos.The Journal of cell biology · 1992Article
- Surface cap modifications in cold-treated Drosophila melanogaster embryos.Cell and tissue research · 1992Article
- Alterations in the establishment and maintenance of epithelial cell polarity as a basis for disease processes.The Journal of clinical investigation · 1990Review
- Identification of microtubule-associated proteins in the centrosome, spindle, and kinetochore of the early Drosophila embryo.The Journal of cell biology · 1989Article
- Article
- Drosophila spectrin: the membrane skeleton during embryogenesis.The Journal of cell biology · 1989Article
- Nuclear and cytoplasmic mitotic cycles continue in Drosophila embryos in which DNA synthesis is inhibited with aphidicolin.The Journal of cell biology · 1988Article
- Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rhodamine-labeled monoclonal antibodies, which react with tyrosinated alpha-tubulin (clone YL 1/2; Kilmartin, J. V., B. Wright, and C. Milstein, 1982, J. Cell Biol., 93:576-582) and label microtubules in vivo (Wehland, J., M. C. Willingham, and I. Sandoval, 1983, J. Cell Biol., 97:1467-1475) were microinjected into syncytial stage Drosophila embryos. At 1 mg/ml antibody concentration, the microtubule arrays of the surface caps became labeled by YL 1/2 but normal development was found to continue. The results are compared with the data from fixed material particularly with regard to interphase microtubules, centrosome separation, and spindle and midbody formation. At 5 mg/ml antibody concentration the microtubules took up larger quantities of antibodies and clumped around the nuclei. Nuclei with clumped microtubules lost their position in the surface layer and moved into the interior. As a result, the F-actin cap meshwork associated with such nuclei either failed to form or subsided. It is concluded that microtubule activity is required to maintain the nuclei in the surface layer and organize the F-actin meshwork of the caps.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.