Evidence map›Paper›PMID 31177508›Full record

ArticleThe journal of physiological sciences : JPS2019

EAE-induced upregulation of mitochondrial MnSOD is associated with increases of mitochondrial SGK1 and Tom20 protein in the mouse kidney cortex.

Sharanpreet Hira, Balamuguran Packialakshmi, Xiaoming Zhou

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of physiological sciences : JPS, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.4field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Sharanpreet HiraDepartment of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD, 20814, USA.
Balamuguran PackialakshmiDepartment of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD, 20814, USA.
Xiaoming ZhouDepartment of Medicine, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD, 20814, USA. xiaoming.zhou@usuhs.edu.ORCID http://orcid.org/0000-0003-4496-4445
Uniformed Services University of the Health Sciences · US

Funding

Henry M. Jackson Foundation MED-83-3923National Heart, Lung, and Blood Institute MED-83-3923National Multiple Sclerosis Society PP3448National Multiple Sclerosis Society Michigan Chapter PP3448
6 · The paper itself

Abstract

Our previous demonstration that severe experimental autoimmune encephalomyelitis (EAE) increases MnSOD protein abundance in the mouse kidney cortex led this study to elucidate the underlying mechanism with monensin-treated HEK293 cells as a model. Severe EAE increases mitochondrial protein abundance of SGK1 kinase and Tom20, a critical subunit of mitochondrial translocase in the renal cortex. In HEK293 cells, catalase inhibits monensin-induced increases of mitochondrial SGK1 and Tom20 protein levels. Further, GSK650394, a specific inhibitor of SGK1 reduces monensin-induced increase of mitochondrial protein abundance of Tom20 and MnSOD. Finally, RNAi of Tom20 reduces the effect of monensin on MnSOD. MnSOD and Tom20 physically associate with each other. In conclusion, in HEK293 cells, mitochondrial reactive oxygen species increase protein abundance of mitochondrial SGK1, which leads to a rise of mitochondrial Tom20, resulting in importing MnSOD protein into the mitochondria. This could be a mechanism by which severe EAE up-regulates mitochondrial MnSOD in the kidney cortex.

Indexed as

AnimalsCatalaseCell LineEncephalomyelitis, Autoimmune, ExperimentalHEK293 CellsHumansImmediate-Early ProteinsKidney CortexMaleMembrane Transport ProteinsMiceMice, Inbred C57BLMitochondriaMitochondrial Precursor Protein Import Complex ProteinsMitochondrial ProteinsProtein Serine-Threonine KinasesCatalaseImmediate-Early ProteinsMembrane Transport ProteinsMitochondrial Precursor Protein Import Complex ProteinsMitochondrial ProteinsProtein Serine-Threonine KinasesReactive Oxygen SpeciesReceptors, Cell SurfaceSerum-Glucocorticoid Regulated KinasesSuperoxide DismutaseTomm20 protein, mouseExperimental autoimmune encephalomyelitisHEK293 cellsMonensinNa,K-ATPaseOuabainReactive oxygen species

Identifiers

PMID31177508
PMCPMC10717134
OpenAlexW2955235999

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.