Evidence map›Paper›PMID 31177431›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Intravital Confocal Microscopy of Dermal Innate Immune Responses to Flea-Transmitted Yersinia pestis.

Jeffrey G Shannon, B Joseph Hinnebusch

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Observational
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Jeffrey G ShannonLaboratory of Bacteriology, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT, USA. jshannon@niaid.nih.gov.
B Joseph HinnebuschLaboratory of Bacteriology, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT, USA.
Rocky Mountain Research (United States) · US

Funding

Animal Models to Study Plague Infection and ImmunityZIAAI000919 · NIAID · NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES · PI HINNEBUSCH, B J · 2009 to 2023
$8.3M
Rodent Models To Study Pathogenesis Of Bubonic And SeptiZ01AI000919 · NIAID · NIAID EXTRAMURAL ACTIVITIES · PI HINNEBUSCH, BERNARD JOSEPH · 2002 to 2008
$1.6M
Intramural NIH HHS Z01 AI000919Intramural NIH HHS ZIA AI000919
6 · The paper itself

Abstract

The technique known as intravital microscopy (IVM), when used in conjunction with transgenic mice expressing fluorescent proteins in various cell populations, is a powerful tool with the potential to provide new insights into host-pathogen interactions in infectious disease pathogenesis in vivo. Yersinia pestis, the causative agent of plague, is typically deposited in a host's skin during feeding of an infected flea. IVM has been used to characterize the innate immune response to Y. pestis in the skin and identify differences between the responses to needle-inoculated and flea-transmitted bacteria that would have been difficult, if not impossible, to detect by other means. Here we describe techniques used to image the neutrophil response to flea-transmitted Y. pestis in the dermis of live mice using conventional confocal microscopy.

Indexed as

Immunity, InnateAnimalsDermisDisease Models, AnimalInsect VectorsIntravital MicroscopyMiceMicroscopy, ConfocalNeutrophilsPlagueSiphonapteraYersinia pestisConfocal microscopyFleaIntravital microscopySkinXenopsylla cheopisYersinia pestis

Identifiers

PMID31177431
PMCPMC11032071
OpenAlexW2955785769

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.