Evidence map›Paper›PMID 31176958›Full record

ArticleDNA repair2019

Quantitative analysis of ATM phosphorylation in lymphocytes.

Christopher J Bakkenist, R Kenneth Czambel, Yan Lin, Nathan A Yates, Xuemei Zeng, Jeffery Shogan, John C Schmitz

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in DNA repair, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 38% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Christopher J BakkenistDepartment of Radiation Oncology, University of Pittsburgh, 5117 Centre Avenue, Pittsburgh, PA, 15213-1863, United States; Department of Pharmacology and Chemical Biology, University of Pittsburgh, 5117 Centre Avenue, Pittsburgh, PA, 15213-1863, United States.
R Kenneth CzambelDepartment of Medicine, University of Pittsburgh School of Medicine, 5117 Centre Avenue, Pittsburgh, PA, 15213-1863, United States.
Yan LinDepartment of Biostatistics, University of Pittsburgh School of Medicine, 5117 Centre Avenue, Pittsburgh, PA, 15213-1863, United States.
Nathan A YatesDepartment of Cell Biology, University of Pittsburgh, 5117 Centre Avenue, Pittsburgh, PA, 15213-1863, United States; Biomedical Mass Spectrometry Center, University of Pittsburgh, 5117 Centre Avenue, Pittsburgh, PA, 15213-1863, United States.
Xuemei ZengBiomedical Mass Spectrometry Center, University of Pittsburgh, 5117 Centre Avenue, Pittsburgh, PA, 15213-1863, United States.
Jeffery ShoganDepartment of Radiation Oncology, University of Pittsburgh, 5117 Centre Avenue, Pittsburgh, PA, 15213-1863, United States.
John C SchmitzDepartment of Medicine, University of Pittsburgh School of Medicine, 5117 Centre Avenue, Pittsburgh, PA, 15213-1863, United States. Electronic address: schmitzjc@upmc.edu.
University of Pittsburgh · US

Funding

VECTOR CORE FACILITYP30CA047904 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Jessie Nedrow · 1988 to 2026
$158.0M
UNIVERSITY OF PITTSBURGH CLINICAL AND TRANSLATIONAL SCIENCE INSTITUTE: BPCAUL1RR024153 · NCRR · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI REIS, STEVEN E · 2006 to 2011
$73.9M
University of Pittsburgh Clinical and Translational Science InstituteUL1TR000005 · NCATS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI REIS, STEVEN E · 2012 to 2015
$50.8M
NCI ET-CTN with Phase i Emphasis at UPCIUM1CA186690 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI JOHN C. BYRD, Farshid Dayyani · 2014 to 2026
$9.1M
DNA damage signaling to immune checkpointsR01CA204173 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BAKKENIST, CHRISTOPHER J. · 2017 to 2021
$1.8M
DNA damage signaling to dormant origins of replicationR01CA236367 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BAKKENIST, CHRISTOPHER J. · 2020 to 2024
$1.8M
NCATS NIH HHS UL1 TR000005NCI NIH HHS P30 CA047904NCI NIH HHS R01 CA204173NCI NIH HHS R01 CA236367NCI NIH HHS UM1 CA186690NCRR NIH HHS UL1 RR024153
6 · The paper itself

Abstract

Since many anticancer therapies target DNA and DNA damage response pathways, biomarkers of DNA damage endpoints may prove valuable in basic and clinical cancer research. Ataxia telangiectasia-mutated (ATM) kinase is the principal regulator of cellular responses to DNA double-strand breaks (DSBs). In humans, ATM autophosphorylation at serine 1981 (p-S1981) is an immediate molecular response to nascent DSBs and ionizing radiation (IR). Here we describe the analytical characteristics and fit-for-purpose validation of a quantitative dual-labeled immunoblot that simultaneously measures p-S1981-ATM and pan-ATM in human peripheral blood mononuclear cells (PBMCs) following ex vivo exposure to 2 Gy IR, facilitating the calculation of %p-ATM. To validate our assay, we isolated PBMCs from 41 volunteers. We report that the median basal level of p-S1981-ATM and pan-ATM was 2.4 and 49.5 ng/10

Indexed as

Protein Processing, Post-TranslationalAtaxia Telangiectasia Mutated ProteinsDNADNA Breaks, Double-StrandedDNA RepairFemaleHumansImmunoblottingLeukocytes, MononuclearLymphocytesMalePhosphorylationRadiation, IonizingReproducibility of ResultsAtaxia Telangiectasia Mutated ProteinsATM protein, humanDNAAtaxia telangiectasia-mutated kinaseBiomarkerDNA damage responsePBMCSex as a biological variable

Identifiers

PMID31176958
PMCPMC6708498
OpenAlexW2948551733

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.