ArticleMicrobial cell factories2019
Esterification of geraniol as a strategy for increasing product titre and specificity in engineered Escherichia coli.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 55 citations in OpenAlex.
- Elucidating the biochemical properties of limonene synthase from Agastache rugosa.BMC plant biology · 2026Article
- Harnessing Pelargonium Odoratissimum Extracts for Sustainable Ground Meat Preservation.Journal of food science · 2025Article
- Sustainable Production of Bio-Based Geraniol: Heterologous Expression of Early Terpenoid Pathway Enzymes inACS synthetic biology · 2025Article
- Production of Esters inMicroorganisms · 2024Article
- Decoding Catalysis by Terpene Synthases.ACS catalysis · 2023Review
- Precursor prioritization for p-cymene production through synergistic integration of biology and chemistry.Biotechnology for biofuels and bioproducts · 2022Article
- Predictive Engineering of Class I Terpene Synthases Using Experimental and Computational Approaches.Chembiochem : a European journal of chemical biology · 2022Review
- Metabolic engineering of Escherichia coli for efficient biosynthesis of butyl acetate.Microbial cell factories · 2022Article
- Alternative metabolic pathways and strategies to high-titre terpenoid production inNatural product reports · 2022Review
- Bioproduction of Linalool From Paper Mill Waste.Frontiers in bioengineering and biotechnology · 2022Article
- Natural Compounds: A Hopeful Promise as an Antibiofilm Agent AgainstFrontiers in pharmacology · 2022Review
- Separation and purification of plant terpenoids from biotransformation.Engineering in life sciences · 2021Review
- Functional Characterization of aInternational journal of molecular sciences · 2020Article
- One Hundred Faces of Geraniol.Molecules (Basel, Switzerland) · 2020Review
- A Gram-Scale Limonene Production Process with EngineeredMolecules (Basel, Switzerland) · 2020Article
- Production of Terpenoids by Synthetic Biology Approaches.Frontiers in bioengineering and biotechnology · 2020Review
- Strategies for the production of biochemicals in bioenergy crops.Biotechnology for biofuels · 2020Review
- Microbial biosynthesis of lactate esters.Biotechnology for biofuels · 2019Article
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5 authors at 1 institution in 1 country.
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Abstract
backgroundGeraniol, an acyclic monoterpene alcohol, is found as a primary constituent in the essential oils of plants such as geranium, lemongrass and rose. The floral-like scent of geraniol has made it a popular constituent of flavour and fragrance products. Over recent decades biotechnology has made significant progress towards the development of industrial platforms for the production of commercially valuable monoterpenoids, such as geraniol, through expression of recombinant terpene biosynthetic pathways in microbial hosts. Titres, however, have been hindered due to the inherent toxicity of these compounds-which are often utilised for anti-microbial and anti-fungal functions in their host plant.
resultsIn this study we modified an Escherichia coli strain, engineered to express a heterologous mevalonate pathway, by replacement of the terpene synthase with a geraniol synthase from Ocimum basilicum for the production of geraniol, and co-expressed an alcohol acyltransferase (AAT) from Rosa hybrida for the specific acetylation of geraniol. The low water solubility of geranyl acetate facilitated its partition into the organic phase of a two-phase system, relieving the cellular toxicity attributed to the build-up of geraniol in the aqueous phase. In a partially optimised system this strain produced 4.8 g/L geranyl acetate (based on the aqueous volume) which, on a molar equivalent basis, represents the highest monoterpene titre achieved from microbial culture to date. It was also found that esterification of geraniol prevented bioconversion into other monoterpenoids, leading to a significant improvement in product specificity, with geranyl acetate being the sole product observed.
conclusionIn this study we have shown that it is possible to both overcome the toxicity limit impeding the production of the monoterpene alcohol geraniol and mitigate product loss in culture through endogenous metabolism by using an in vivo esterification strategy. This strategy has resulted in the highest geraniol (equivalent) titres achieved from a microbial host, and presents esterification as a viable approach to increasing the titres obtained in microbial monoterpenoid production.
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