Evidence map›Paper›PMID 31175472›Full record

ArticleExperimental & applied acarology2019

Molecular identification of spotted fever group Rickettsia in ticks collected from dogs and small ruminants in Greece.

Alberto Moraga-Fernández, Ιlias Chaligiannis, Alejandro Cabezas-Cruz, Anna Papa, Smaragda Sotiraki, José de la Fuente, Isabel G Fernández de Mera

Abstract read
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In one paragraph

Article in Experimental & applied acarology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.2field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Molecular Survey ofVeterinary sciences · 2023
    Article
  7. Molecular Detection ofVeterinary sciences · 2023
    Article
  8. Article
  9. Spotted Fever GroupVeterinary sciences · 2022
    Article
  10. Review
  11. Preliminary Evidence ofPathogens (Basel, Switzerland) · 2021
    Article
  12. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 7 countries.

Alberto Moraga-FernándezSaBio, Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo, 12, 13005, Ciudad Real, Spain.
Ιlias ChaligiannisVeterinary Research Institute, Hellenic Agricultural Organization Demeter, Thermi, 57001, Thessaloniki, Greece.ORCID http://orcid.org/0000-0002-8224-5911
Alejandro Cabezas-CruzUMR BIPAR, INRA, ANSES, Ecole Nationale Vétérinaire d'Alfort, Université Paris-Est, Maisons-Alfort, France.ORCID http://orcid.org/0000-0002-8660-730X
Anna PapaDepartment of Microbiology, Medical School, Aristotle University of Thessaloniki, Thessaloniki, Greece.ORCID http://orcid.org/0000-0002-1032-2965
Smaragda SotirakiVeterinary Research Institute, Hellenic Agricultural Organization Demeter, Thermi, 57001, Thessaloniki, Greece.
José de la FuenteSaBio, Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo, 12, 13005, Ciudad Real, Spain.ORCID http://orcid.org/0000-0001-7383-9649
Isabel G Fernández de MeraSaBio, Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC-UCLM-JCCM), Ronda de Toledo, 12, 13005, Ciudad Real, Spain. mariaisabel.garcia@uclm.es.ORCID http://orcid.org/0000-0001-5936-4018
Aristotle University of Thessaloniki · GRInstituto de Investigación en Recursos Cinegéticos · ESCentre for Research and Technology Hellas · GRÉcole Nationale Vétérinaire d'Alfort · FROklahoma State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spotted fever group (SFG) Rickettsia are zoonotic and emerging pathogens with considerable impact in public and animal health. Greece is an endemic country of diseases caused by SFG Rickettsia. This work aims to evaluate the prevalence of SFG Rickettsia in ticks collected from domestic hosts including sheep, goats and dogs. Several genetic markers for bacterial genes, such as 16S rRNA, ompA, ompB, atpA, gltA, recA, dnaA and dnaK, were amplified and sequenced to accurately classified the rickettsial pathogens in the ticks. Taxonomy and species classification of the Rickettsia was achieved by combining phylogenetic and in silico digestion analysis of the gene sequences obtained. A total of 187 ticks were collected and classified at the species level as Ixodes gibosus, Dermacentor marginatus, Haemaphysalis parva, H. sulcata, H. punctata, Hyalomma scavatum, Rhipicephalus sanguineus, R. bursa and Rhipicephalus sp. The results showed that 7.5% of ticks were infected with at least one SFG Rickettsia including R. massiliae (n = 3), R. slovaca (n = 5), R. raoultii (n = 1) and R. hoogstraalii (n = 5), collected from sheep (n = 4), goats (n = 5) and dogs (n = 3). Molecular analysis revealed the presence of novel genetic variants of R. hoogstraalii (in H. sulcata and H. parva from goat and sheep) and R. raoultii (in D. marginatus from goat). These results proof the presence of SFG Rickettsia in domestic hosts in Greece, and support the need for continued monitoring, surveillance and further analyses of other hosts and study areas.

Indexed as

AnimalsDogsFemaleGoatsGreeceIxodidaeLarvaMalePhylogenyRickettsiaSheepGreeceSpotted fever group RickettsiaTick-borne diseasesTicks

Identifiers

PMID31175472
OpenAlexW2948666000

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.