ArticleNature protocols2019
Solvent-assisted preparation of supported lipid bilayers.
Article in Nature protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The trial behind it
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Who cites it
46 citing papers in PubMed.
- The Myelin Sheath as a Multilamellar Electromechanical System: Bilayer Stack Analogs, Energy Buffering, and Biological Memory.Membranes · 2026Article
- A Solvent-Assisted Supported Bilayer QCM-D Platform for Resolving Composition- and Structure-Dependent Antimicrobial Peptide-Membrane Interactions.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Bottom-up reconstitution of model membrane systems: a mini-review.Biochemical Society transactions · 2026Review
- Structure and composition of supported lipid bilayers: a comparison between solvent-assisted lipid bilayer and vesicle fusion methods.Journal of applied crystallography · 2026Article
- 4polar3D single molecule imaging of 3D orientation in dense actin networks using ratiometric polarization splitting.Nature communications · 2026Article
- Synergistic Effect of Paclitaxel and Epirubicin Coadministration─Insight into the Mechanisms of Interactions with Model Breast Cancer Cell Membranes.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Solid-Supported Polymer Membranes: How Different Deposition Methods Influence Their Inner Morphology and Properties.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- OPSALC: On-Particle Solvent-Assisted Lipid Coating to Create Erythrocyte Membrane-like Coatings with Improved Hemocompatibility.ACS applied materials & interfaces · 2025Article
- Phase-separating peptide coacervates with programmable material properties for universal intracellular delivery of macromolecules.Nature communications · 2024Article
- Chaotropic Agent-Assisted Supported Lipid Bilayer Formation.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Assessing the Quality of Solvent-Assisted Lipid Bilayers Formed at Different Phases and Aqueous Buffer Media: A QCM-D Study.Sensors (Basel, Switzerland) · 2024Article
- Advancements in Engineering Planar Model Cell Membranes: Current Techniques, Applications, and Future Perspectives.Nanomaterials (Basel, Switzerland) · 2024Review
- Cell-free expression with a quartz crystal microbalance enables rapid, dynamic, and label-free characterization of membrane-interacting proteins.Communications biology · 2024Article
- Concealing Organic Neuromorphic Devices with Neuronal-Inspired Supported Lipid Bilayers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Emerging Designs and Applications for Biomembrane Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2024Review
- Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform.Nature communications · 2024Article
- A Label-Free Multitechnique Approach to Characterize the Interaction of Bioactive Compounds with Biomimetic Interfaces.Small science · 2024Article
- Cholesterol-Enriched Hybrid Lipid Bilayer Formation on Inverse Phosphocholine Lipid-Functionalized Titanium Oxide Surfaces.Biomimetics (Basel, Switzerland) · 2023Article
- Structural and functional insights into the delivery of a bacterial Rhs pore-forming toxin to the membrane.Nature communications · 2023Article
- Molecular fingerprinting of biological nanoparticles with a label-free optofluidic platform.Research square · 2023Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The supported lipid bilayer (SLB) platform is a popular cell membrane mimic that is utilized in the chemical, biological, materials science, and medical fields. To date, SLB preparation has proven challenging because of the need for specialized fabrication equipment, domain-specific knowledge about topics relevant to lipid self-assembly, and extensive training in the interfacial science field. Existing methods, such as vesicle fusion, also work with only a narrow range of lipid compositions and material supports. Here, we describe a recently developed simple and versatile protocol to form SLBs. The protocol is simple because it requires minimal sample preparation and only basic microfluidics, making it technically accessible to researchers across different scientific disciplines. The protocol is versatile because it works on a wide range of material supports, such as silicon oxide, gold, and graphene, and is compatible with diverse lipid compositions, including sterols and signaling lipids. The main stages of the procedure involve dissolving a lipid sample in an organic solvent, depositing the lipid solution on a solid support, and replacing the organic solvent with aqueous buffer. In addition, we provide procedures for characterizing the quality of the prepared SLBs and present examples of biofunctionalization procedures. The protocol takes 1-2 h and is broadly useful in various application contexts, including clinical diagnostics, biosensing, and cellular interfaces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.