Evidence map›Paper›PMID 31175034›Full record

ReviewCurrent opinion in structural biology2019

Enabling technologies in super-resolution fluorescence microscopy: reporters, labeling, and methods of measurement.

Alecia Marie Achimovich, Huiwang Ai, Andreas Gahlmann

Abstract readReview
In one paragraph

Review in Current opinion in structural biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Alecia Marie AchimovichDepartment of Molecular Physiology & Biological Physics, University of Virginia School of Medicine, Charlottesville, VA, USA.
Huiwang AiDepartment of Molecular Physiology & Biological Physics, University of Virginia School of Medicine, Charlottesville, VA, USA; Department of Chemistry, University of Virginia, Charlottesville, VA, USA.
Andreas GahlmannDepartment of Molecular Physiology & Biological Physics, University of Virginia School of Medicine, Charlottesville, VA, USA; Department of Chemistry, University of Virginia, Charlottesville, VA, USA. Electronic address: agahlmann@virginia.edu.

Funding

Training in Molecular BiophysicsT32GM080186 · NIGMS · UNIVERSITY OF VIRGINIA · PI NAKAMOTO, ROBERT K. · 2008 to 2019
$1.5M
A Fluorescent Toolkit for Imaging Reactive Oxygen Species (ROS)R01GM118675 · NIGMS · UNIVERSITY OF VIRGINIA · PI AI, HUIWANG · 2016 to 2019
$1.4M
NIGMS NIH HHS R01 GM118675NIGMS NIH HHS T32 GM080186
6 · The paper itself

Abstract

Super-resolution fluorescence microscopy continues to experience a period of extraordinary development. New instrumentation and fluorescent labeling strategies provide access to molecular and cellular processes that occur on length scales ranging from nanometers to millimeters and on time scales ranging from milliseconds to hours. At the shortest length scales, single-molecule imaging methods now allow measurement of nanoscale localization, motion, and binding kinetics of individual biomolecules. At cellular and intercellular length scales, super-resolution microscopy allows structural and functional imaging of individual cells in tissues and even in whole animals. Here, we review recent advances that have enabled entirely new types of experiments and greatly potentiated existing technologies.

Indexed as

AnimalsHumansMicroscopy, FluorescenceSignal-To-Noise RatioSingle Molecule Imaging

Identifiers

PMID31175034
PMCPMC6778497

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.