ArticleBMC veterinary research2019
Metabolomic studies as a tool for determining the post-mortem interval (PMI) in stillborn calves.
Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Advances in postmortem interval estimation: A systematic review of machine learning and metabolomics across various tissue types.Forensic science, medicine, and pathology · 2025Pooled it
- Identification of candidate blood biomarkers through metabolomics analysis in bovine superovulation.Frontiers in veterinary science · 2025Article
- Influence of Drugs and Toxins on Decomposition Dynamics: Forensic Implications.Molecules (Basel, Switzerland) · 2024Review
- A fundamental study on postmortem submersion interval estimation by metabolomics analyzing of gastrocnemius muscle from submersed rat models in freshwater.International journal of legal medicine · 2024Article
- Postmortem Alteration of Purine Metabolism in Coronary Artery Disease.Metabolites · 2023Article
- Estimating the time of human decomposition based on skeletal muscle biopsy samples utilizing an untargeted LC-MS/MS-based proteomics approach.Analytical and bioanalytical chemistry · 2023Article
- Article
- In Vitro Animal Model for Estimating the Time since Death with Attention to Early Postmortem Stage.Metabolites · 2022Article
- A preliminary study on early postmortem submersion interval (PMSI) estimation and cause-of-death discrimination based on nontargeted metabolomics and machine learning algorithms.International journal of legal medicine · 2022Article
- Combining with lab-on-chip technology and multi-organ fusion strategy to estimate post-mortem interval of rat.Frontiers in medicine · 2022Article
- The Application of Metabolomics in Forensic Science with Focus on Forensic Toxicology and Time-of-Death Estimation.Metabolites · 2021Review
- Changes of Metabolites and Gene Expression under Different Feeding Systems Associated with Lipid Metabolism in Lamb Meat.Foods (Basel, Switzerland) · 2021Article
- Role of Infection and Immunity in Bovine Perinatal Mortality: Part 2. Fetomaternal Response to Infection and Novel Diagnostic Perspectives.Animals : an open access journal from MDPI · 2021Review
- Forensic NMR metabolomics: one more arrow in the quiver.Metabolomics : Official journal of the Metabolomic Society · 2020Review
- Comparative metabolome analysis of serum changes in sheep under overgrazing or light grazing conditions.BMC veterinary research · 2019Article
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6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundPerinatal mortality may vary between herds, but the cost of deaths are always higher than value of the calf. When diagnosing the cause of a calf's death it is important to determine when it occurred, before or after calving. Metabolomics is widely used to identify many human diseases, but quite rarely applied in veterinary science. The aim of this study was to compare the metabolic profiles of calves with different times of death and those of calves born alive. Into the study, twenty one healthy controls (singleton, normal assisted calving, born alive) and 75 stillborn (SB) calves (with a gestation length of ≥260 days, SB, or dead within 6 h of birth) were enrolled. Plasma and urine from SB and control calves were investigated by proton nuclear magnetic resonance based metabolomic methods. SB calves were divided into four PMI groups. One PMI group included calves that died after calving and the other groups - three comprised in utero deaths, based on pathophysiological changes (lung inflation, autolysis in internal organs, hemoglobin imbibition in the pleura and aortic arch). Partial Least Squares - Discriminant Analysis models based on plasma metabolites were calculated, reflecting assumed data clustering.
resultsTwenty six metabolites in plasma and 29 in urine changed significantly with PMI according to one way analysis of variance. Half the metabolites in plasma and the majority in urine increased with PMI. Six metabolites increased simultaneously in plasma and urine: acetate, sn-glycero-3-phosphocholine (GPC), leucine, valine, creatine, and alanine.
conclusionsPost-mortem changes in calves were associated with molecular variations in blood plasma and urine, showing the greatest differences for the group in which the post-mortem pathological changes were the most advanced. The results of the study show that evaluation of calf plasma or urine may be used as a diagnostic method for the determination of the PMI. Moreover, the metabolites, which unambiguously increased or decreased, can be used as potential biomarkers of PMI.
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