ArticleJournal of molecular cell biology2020
Mitotic motor CENP-E cooperates with PRC1 in temporal control of central spindle assembly.
Article in Journal of molecular cell biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 32 citations in OpenAlex.
- Probing Centromere Plasticity and Chromatin Remodeling Using Single-Molecule Techniques.Cell biology international · 2026Review
- Condensation of MATCAP promotes CENP-E-dependent chromosome congression in mitosis.Nature communications · 2026Article
- Biomimetic fusion nanosystem from ginger exosomes and tumor cell membranes: boosting PLK1-targeted therapy in BRCA-heterogeneous HGSOC.Journal of nanobiotechnology · 2026Article
- Mitotic Machinery Dysregulation in Lung Cancer: Biological Roles, Therapeutic Targeting, and Combination Strategies.Pharmaceutics · 2026Review
- CENP-E initiates chromosome congression by opposing Aurora kinases to promote end-on attachments.Nature communications · 2025Article
- Global landscape of hepatic organoid research: A bibliometric and visual study.World journal of hepatology · 2025Article
- Article
- Modeling gastric intestinal metaplasia in 3D organoids using nitrosoguanidine.Journal of molecular cell biology · 2024Article
- Sgo1 interacts with CENP-A to guide accurate chromosome segregation in mitosis.Journal of molecular cell biology · 2024Article
- Kinesin-7 CENP-E in tumorigenesis: Chromosome instability, spindle assembly checkpoint, and applications.Frontiers in molecular biosciences · 2024Review
- Phosphorylation controls spatial and temporal activities of motor-PRC1 complexes to complete mitosis.The EMBO journal · 2023Article
- Dynamics and regulation of mitotic chromatin accessibility bookmarking at single-cell resolution.Science advances · 2023Article
- Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assembly.Nature communications · 2022Article
- Syntelin inhibits triple-negative breast cancer cell proliferation and metastasis.Journal of molecular cell biology · 2022Article
- Dynamic crotonylation of EB1 by TIP60 ensures accurate spindle positioning in mitosis.Nature chemical biology · 2021Article
- The septin complex links the catenin complex to the actin cytoskeleton for establishing epithelial cell polarity.Journal of molecular cell biology · 2021Article
- Potent antitumor activity of a glutamyltransferase-derived peptide via an activation of oncosis pathway.Scientific reports · 2021Article
- Mechanisms and regulation underlying membraneless organelle plasticity control.Journal of molecular cell biology · 2021Review
- PS-341 alleviates chronic low-grade inflammation and improves insulin sensitivity through the inhibition of TM4 (UBAC2) degradation.Nutrition & metabolism · 2021Article
- Phase separation drives decision making in cell division.The Journal of biological chemistry · 2020Review
Corrections and comments
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Authors and funding
22 authors at 2 institutions in 2 countries.
Funding
Abstract
Error-free cell division depends on the accurate assembly of the spindle midzone from dynamic spindle microtubules to ensure chromatid segregation during metaphase-anaphase transition. However, the mechanism underlying the key transition from the mitotic spindle to central spindle before anaphase onset remains elusive. Given the prevalence of chromosome instability phenotype in gastric tumorigenesis, we developed a strategy to model context-dependent cell division using a combination of light sheet microscope and 3D gastric organoids. Light sheet microscopic image analyses of 3D organoids showed that CENP-E inhibited cells undergoing aberrant metaphase-anaphase transition and exhibiting chromosome segregation errors during mitosis. High-resolution real-time imaging analyses of 2D cell culture revealed that CENP-E inhibited cells undergoing central spindle splitting and chromosome instability phenotype. Using biotinylated syntelin as an affinity matrix, we found that CENP-E forms a complex with PRC1 in mitotic cells. Chemical inhibition of CENP-E in metaphase by syntelin prevented accurate central spindle assembly by perturbing temporal assembly of PRC1 to the midzone. Thus, CENP-E-mediated PRC1 assembly to the central spindle constitutes a temporal switch to organize dynamic kinetochore microtubules into stable midzone arrays. These findings reveal a previously uncharacterized role of CENP-E in temporal control of central spindle assembly. Since CENP-E is absent from yeast, we reasoned that metazoans evolved an elaborate central spindle organization machinery to ensure accurate sister chromatid segregation during anaphase and cytokinesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.