Evidence map›Paper›PMID 31173689›Full record

ArticleACS nano2019

Assembly Reactions of Hepatitis B Capsid Protein into Capsid Nanoparticles Follow a Narrow Path through a Complex Reaction Landscape.

Roi Asor, Lisa Selzer, Christopher John Schlicksup, Zhongchao Zhao, Adam Zlotnick, Uri Raviv

Abstract read
In one paragraph

Article in ACS nano, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed.

  1. Article
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  10. Upgrade ofJournal of applied crystallography · 2023
    Article
  11. Article
  12. Article
  13. Mechanism of Tubulin Oligomers and Single-Ring Disassembly Catastrophe.The journal of physical chemistry letters · 2022
    Article
  14. Article
  15. Protein assembly and crowding simulations.Current opinion in structural biology · 2022
    Review
  16. Article
  17. Review
  18. Article
  19. Physics of viral dynamics.Nature reviews. Physics · 2021
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Roi AsorInstitute of Chemistry and the Center for Nanoscience and Nanotechnology , The Hebrew University of Jerusalem , Edmond J. Safra Campus, Givat Ram, Jerusalem 9190401 , Israel.
Lisa SelzerDepartment of Molecular and Cellular Biochemistry , Indiana University , Bloomington , Indiana 47405 , United States.
Christopher John SchlicksupDepartment of Molecular and Cellular Biochemistry , Indiana University , Bloomington , Indiana 47405 , United States.
Zhongchao ZhaoDepartment of Molecular and Cellular Biochemistry , Indiana University , Bloomington , Indiana 47405 , United States.
Adam ZlotnickDepartment of Molecular and Cellular Biochemistry , Indiana University , Bloomington , Indiana 47405 , United States.ORCID 0000-0001-9945-6267
Uri RavivInstitute of Chemistry and the Center for Nanoscience and Nanotechnology , The Hebrew University of Jerusalem , Edmond J. Safra Campus, Givat Ram, Jerusalem 9190401 , Israel.ORCID 0000-0001-5992-9437

Funding

Multimode Observation of Virus Capsid AssemblyR01AI118933 · NIAID · TRUSTEES OF INDIANA UNIVERSITY · PI Adam Zlotnick · 2016 to 2026
$4.0M
Graduate Program in Quantitative and Chemical Biology at Indiana University BloomT32GM109825 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI GIEDROC, DAVID P. · 2014 to 2018
$926k
NIAID NIH HHS R01 AI118933NIGMS NIH HHS T32 GM109825
6 · The paper itself

Abstract

For many viruses, capsids (biological nanoparticles) assemble to protect genetic material and dissociate to release their cargo. To understand these contradictory properties, we analyzed capsid assembly for hepatitis B virus; an endemic pathogen with an icosahedral, 120-homodimer capsid. We used solution X-ray scattering to examine trapped and equilibrated assembly reactions. To fit experimental results, we generated a library of distinct intermediates, selected by umbrella sampling of Monte Carlo simulations. The number of possible capsid intermediates is immense, ∼10

Indexed as

CapsidCapsid ProteinsEntropyHepatitis B virusMolecular Dynamics SimulationMonte Carlo MethodNanoparticlesCapsid Proteinsdimer−dimer association free energygrand canonical free energy landscape of capsid self-assembly reactionhepatitis b viruskinetically trapped intermediatesmaximum entropy optimizationsmall-angle X-ray scatteringumbrella sampling Monte Carlo

Identifiers

PMID31173689
PMCPMC7243059

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.