Evidence map›Paper›PMID 31173196›Full record

ArticleMolecular medicine reports2019

A comparative study of the effects of concentrated growth factors in two different forms on osteogenesis in vitro.

Liping Wang, Mianjia Wan, Zhengmao Li, Ningying Zhong, Dongliang Liang, Linhu Ge

Registry-linked trialAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular medicine reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07253233 (A Prospective, Single-center, Single-arm Clinical Study Protocol on the Treatment of Spinal Cord Injury Using Autologous Concentrated Growth Factors), which is not on this map. Cited by 23 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
23citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07253233 early_phase1not yet recruitingnot on this mapstarted 2025, after this paper: background citation

A Prospective, Single-center, Single-arm Clinical Study Protocol on the Treatment of Spinal Cord Injury Using Autologous Concentrated Growth Factors

TypeinterventionalSponsorThird Affiliated Hospital, Sun Yat-Sen UniversityRan2025 to 2027Enrolled10ConditionsSpinal Cord InjuryArmsConcentrated Growth Factors (CGF)
3 · Its place in the literature

Who cites it

23 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Freeze-Drying of Platelet-Rich Plasma: The Quest for Standardization.International journal of molecular sciences · 2020
    Pooled it
  2. Trial
  3. Trial
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Evaluation of antibacterial effect of concentrated growth factor onJournal of family medicine and primary care · 2022
    Article
  14. Article
  15. Article
  16. [Research advances on platelet-rich blood products combined with biological materials in wound repair].Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns · 2021
    Article
  17. Review
  18. Review
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Liping WangKey Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China.
Mianjia WanKey Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China.
Zhengmao LiKey Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China.
Ningying ZhongKey Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China.
Dongliang LiangKey Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China.
Linhu GeKey Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Affiliated Stomatology Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510140, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extending the release cycle of growth factors to match the cycle of bone remodeling is difficult. When using concentrated growth factors (CGFs), the release of growth factors is excessively rapid. In the present study, CGF samples were prepared by centrifugation. CGF samples were then lyophilized and grinded into a powder, which was termed freeze‑dried CGF. The freeze‑dried CGF samples were mixed with chitosan‑alginate composite hydrogels, and the mixture was lyophilized. The result was a chitosan‑alginate composite CGF membrane, which was called sustained‑release CGF. This study investigated whether freeze‑dried CGF in a chitosan‑alginate composite gel can release CGF steadily to achieve effective osteogenesis. The proliferation and osteogenic expression of MC3T3‑E1 cells induced by the supernatants from incubation with freeze‑dried CGF and sustained‑release CGF were evaluated. The concentrations of the growth factors, transforming growth factor β1 (TGF‑β1), insulin‑like growth factor‑1 (IGF‑1), platelet‑derived growth factor‑AB (PDGF‑AB) and vascular endothelial growth factor (VEGF), in these two experimental groups at different times were determined by ELISA kits. The freeze‑dried CGF showed better osteogenic performance than the sustained‑release CGF in the early stages. At later stages, the sustained‑release CGF had significant advantages over freeze‑dried CGF in terms of promoting osteogenic mineralization. By characterizing the biologic properties of the CGF in the two different forms in vitro, we obtained a better understanding of their clinical effects.

Indexed as

AdultAlginatesAnimalsCell DifferentiationCell LineCell ProliferationChitosanDelayed-Action PreparationsDrug CompoundingDrug LiberationFemaleFreeze DryingHumansInsulin-Like Growth Factor IKineticsMaleAlginatesChitosanDelayed-Action PreparationsIGF1 protein, humanInsulin-Like Growth Factor IPlatelet-Derived Growth Factorplatelet-derived growth factor ABTGFB1 protein, humanTransforming Growth Factor beta1Vascular Endothelial Growth Factor AVEGFA protein, human

Identifiers

PMID31173196
PMCPMC6625392

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.