ArticleInternational journal of molecular medicine2019
Strontium promotes osteogenic differentiation by activating autophagy via the the AMPK/mTOR signaling pathway in MC3T3‑E1 cells.
Article in International journal of molecular medicine, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 60 citations in OpenAlex.
- Advances in biomimetic mineralized biomaterials for bone tissue engineering.Regenerative biomaterials · 2026Review
- From hard tissues to beyond: Progress and challenges of strontium-containing biomaterials in regenerative medicine applications.Bioactive materials · 2025Review
- Three-dimensional-printed strontium-incorporated β-TCP bioceramic triply periodic minimal surface scaffolds with enhanced angiogenic and osteogenic properties.Regenerative biomaterials · 2025Article
- A Golden Shield: The Protective Role of Curcumin against Liver Fibrosis.Current medicinal chemistry · 2025Review
- Psoralen-mediated regulation of osteogenic differentiation of periodontal ligament stem cells: involvement of the mTOR pathway.Frontiers in cell and developmental biology · 2025Article
- Strategic incorporation of metal ions in bone regenerative scaffolds: multifunctional platforms for advancing osteogenesis.Regenerative biomaterials · 2025Review
- PMAIP1 regulates autophagy in osteoblasts via the AMPK/mTOR pathway in osteoporosis.Human cell · 2024Article
- Effect of strontium on transcription factors identified by transcriptome analyses of bovine ruminal epithelial cells.BMC veterinary research · 2024Article
- Research progresses on mitochondrial-targeted biomaterials for bone defect repair.Regenerative biomaterials · 2024Review
- COPI Vesicle Disruption Inhibits Mineralization via mTORC1-Mediated Autophagy.International journal of molecular sciences · 2023Article
- A Comparative Study on Physicochemical Properties andACS applied bio materials · 2023Article
- The Role of Trace Elements and Minerals in Osteoporosis: A Review of Epidemiological and Laboratory Findings.Biomolecules · 2023Review
- Brevilin A inhibits RANKL-induced osteoclast differentiation and bone resorption.In vitro cellular & developmental biology. Animal · 2023Article
- Advanced applications of strontium-containing biomaterials in bone tissue engineering.Materials today. Bio · 2023Review
- AMPK/mTOR Pathway Is Involved in Autophagy Induced by Magnesium-Incorporated TiOJournal of functional biomaterials · 2022Article
- Cholesterol inhibits autophagy in RANKL-induced osteoclast differentiation through activating the PI3K/AKT/mTOR signaling pathway.Molecular biology reports · 2022Article
- Article
- Probucol promotes osteoblasts differentiation and prevents osteoporosis development through reducing oxidative stress.Molecular medicine (Cambridge, Mass.) · 2022Article
- A Highly Selective GSK-3β Inhibitor CHIR99021 Promotes Osteogenesis by Activating Canonical and Autophagy-Mediated Wnt Signaling.Frontiers in endocrinology · 2022Article
- Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Strontium (Sr) is an alkaline earth metal that exerts the dual effect of improving bone formation and suppressing bone resorption, resulting in increased bone apposition rates and bone mineral density. However, the mechanisms through which Sr exerts these beneficial effects on bone have yet to be fully elucidated. The present study aimed to reveal the underlying molecular mechanisms associated with Sr‑induced osteogenic differentiation. The effects of Sr on cell proliferation and osteogenic differentiation were analyzed by MTT assay, RT‑qPCR, western blot analysis, alkaline phosphatase (ALP) and Alizarin red staining assays. The extent of autophagy was determined by monodansylcadaverine (MDC) staining and western blot analysis of two markers of cellular autophagic activity, the steatosis‑associated protein, sequestosome‑1 (SQSTM1/p62), and the two isoforms of microtubule‑associated protein 1 light chain 3 (LC3), LC‑3‑I/II. The expression levels of AMP‑activated protein kinase (AMPK) and mammalian target of rapamycin (mTOR) were also detected by western blot analysis. Sr at a concentration of 3 mM exerted the most pronounced effect on osteogenic differentiation, without any apparent cell toxicity. At the same time, cellular autophagy was active during this process. Subsequently, autophagy was blocked by 3‑methyladenine, and the enhancement of osteogenic differentiation in response to Sr was abrogated. Additionally, the phosphorylation level of AMPK was significantly increased, whereas that of mTOR was significantly decreased, in the Sr‑treated group. Taken together, the findings of the present study demonstrate that Sr stimulates AMPK‑activated autophagy to induce the osteogenic differentiation of MC3T3‑E1 cells.
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