Evidence map›Paper›PMID 31172482›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2019

Hydro-Cy3-Mediated Detection of Reactive Oxygen Species In Vitro and In Vivo.

Bejan J Saeedi, Bindu Chandrasekharan, Andrew S Neish

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Bejan J SaeediDepartment of Pathology, Emory University School of Medicine, Atlanta, GA, USA.
Bindu ChandrasekharanDepartment of Pathology, Emory University School of Medicine, Atlanta, GA, USA.
Andrew S NeishDepartment of Pathology, Emory University School of Medicine, Atlanta, GA, USA. aneish@emory.edu.
Emory University · US

Funding

Microbial Influence on Mucosal HomeostasisR01AI064462 · NIAID · EMORY UNIVERSITY · PI NEISH, ANDREW S · 2006 to 2022
$5.5M
Control of Epithelial Proliferation by the MicrobiotaR01CA179424 · NCI · EMORY UNIVERSITY · PI JONES, RHEINALLT MELFYN, NEISH, ANDREW S · 2014 to 2017
$1.3M
Epithelial specific ubiquitination eventsR56AI064462 · NIAID · EMORY UNIVERSITY · PI NEISH, ANDREW S · 2011 to 2011
$379k
NCI NIH HHS R01 CA179424NIAID NIH HHS R01 AI064462NIAID NIH HHS R56 AI064462
6 · The paper itself

Abstract

Reactive oxygen species (ROS) are potent signaling molecules with critical roles in cellular pathology and homeostasis. They are produced in all cell types via a diverse array of cellular machinery, giving rise to an equally diverse repertoire of molecular effects. These range from cytotoxic killing of microbes to alteration of the cellular transcriptional response to stress. Despite their importance, research into ROS has been difficult given their inherent instability and transient signaling properties. Herein we describe methods for the use of the redox-sensitive probe hydro-Cy3 for the detection and quantification of ROS both in vitro and in vivo.

Indexed as

Molecular ImagingAnimalsCarbocyaninesCell Membrane PermeabilityFluorescent DyesMiceMicroscopy, ConfocalMolecular StructureNADPH OxidasesOxidation-ReductionOxidative StressReactive Oxygen SpeciesCarbocyaninescyanine dye 3Fluorescent DyesNADPH OxidasesReactive Oxygen SpeciesHydro-Cy3NADPH oxidaseNOXOxidative stressReactive oxygen speciesROS

Identifiers

PMID31172482
PMCPMC12199619
OpenAlexW2964312313

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.