Evidence map›Paper›PMID 31171997›Full record

ArticleTranslational vision science & technology2019

Transplantation of Mature Photoreceptors in Rodents With Retinal Degeneration.

Henri Lorach, Seungbum Kang, Mohajeet B Bhuckory, Alix Trouillet, Roopa Dalal, Michael Marmor, Daniel Palanker

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational vision science & technology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 16 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Annals of translational medicine · 2020
    Review
  8. Article
  9. Development of Stem Cell Therapies for Retinal Degeneration.Cold Spring Harbor perspectives in biology · 2020
    Review
  10. Review
  11. Article
  12. Review
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Henri LorachHansen Experimental Physics Laboratory, Stanford University, CA, USA.
Seungbum KangHansen Experimental Physics Laboratory, Stanford University, CA, USA.
Mohajeet B BhuckoryHansen Experimental Physics Laboratory, Stanford University, CA, USA.
Alix TrouilletDepartment of Otolaryngology, Stanford University, CA, USA.
Roopa DalalDepartment of Ophthalmology, Stanford University, CA, USA.
Michael MarmorDepartment of Ophthalmology, Stanford University, CA, USA.
Daniel PalankerHansen Experimental Physics Laboratory, Stanford University, CA, USA.
Stanford University · USCatholic University of Korea · KR

Funding

Stanford Vision Research CoreP30EY026877 · NEI · STANFORD UNIVERSITY · PI Jeffrey L Goldberg · 2017 to 2026
$8.0M
NEI NIH HHS P30 EY026877
6 · The paper itself

Abstract

purposeTo demonstrate survival and integration of mature photoreceptors transplanted with the retinal pigment epithelium (RPE).

methodsFull-thickness retina with attached RPE was harvested from healthy adult rats. Grafts were implanted into two rat models of retinal degeneration, Royal College of Surgeons (RCS) and S334ter-3. Survival of the host and transplanted retina was monitored using optical coherence tomography (OCT) for up to 6 months. The retinal structure and synaptogenesis between the host and transplant was assessed by histology and immunohistochemistry.

resultsOCT and histology demonstrated a well-preserved photoreceptor layer with inner and outer segments, while the inner retinal layers of the transplant largely disappeared. Grafts, including RPE, survived better than without and the transplanted RPE appeared as a monolayer integrated with the native one. Synaptogenesis was observed through sprouting of new dendrites from the host bipolar cells and synaptic connections forming with cells of the transplant. However, in many samples, a glial fibrillary acidic protein-positive membrane separated the host retina and the graft.

conclusionsPresence of RPE in the graft improved the survival of transplanted photoreceptors. Functional integration between the transplant and the host retina is likely to be further enhanced if formation of a glial seal could be prevented. Transplantation of the mature photoreceptors with RPE may be a practical approach to restoration of sight in retinal degeneration. TRANSLATIONAL RELEVANCE: This approach to restoration of sight in patients with photoreceptor degeneration can be rapidly advanced to clinical testing. In patients with central scotoma, autologous transplantation of the peripheral retina can be an option.

Indexed as

photoreceptor transplantationretinal degenerationRPE transplantation

Identifiers

PMID31171997
PMCPMC6543858
OpenAlexW2946891913

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.