Evidence map›Paper›PMID 31171994›Full record

ArticleTranslational vision science & technology2019

Extracellular Matrix Protein Coating of Processed Fish Scales Improves Human Corneal Endothelial Cell Adhesion and Proliferation.

Yi-Jen Hsueh, David Hui-Kang Ma, Kathleen Sheng-Chuan Ma, Tze-Kai Wang, Cheng-Hung Chou, Chien-Cheng Lin, Min-Chang Huang, Li-Jyuan Luo, Jui-Yang Lai, Hung-Chi Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational vision science & technology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 1 country.

Yi-Jen HsuehLimbal Stem Cell Laboratory, Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou, Taiwan.
David Hui-Kang MaLimbal Stem Cell Laboratory, Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou, Taiwan.
Kathleen Sheng-Chuan MaLimbal Stem Cell Laboratory, Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou, Taiwan.
Tze-Kai WangLimbal Stem Cell Laboratory, Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou, Taiwan.
Cheng-Hung ChouDepartment of Research, Body Organ Biomedical Corporation, Taipei, Taiwan.
Chien-Cheng LinDepartment of Research, Body Organ Biomedical Corporation, Taipei, Taiwan.
Min-Chang HuangDepartment of Research, Body Organ Biomedical Corporation, Taipei, Taiwan.
Li-Jyuan LuoDepartment of Chemical and Materials Engineering, Chang Gung University, Taoyuan, Taiwan.
Jui-Yang LaiCenter for Tissue Engineering, Chang Gung Memorial Hospital, Linkou, Taiwan.
Hung-Chi ChenLimbal Stem Cell Laboratory, Department of Ophthalmology, Chang Gung Memorial Hospital, Linkou, Taiwan.
Body Organ Biomedical (Taiwan) · TWChang Gung Memorial Hospital · TWChang Gung University · TWMing Chi University of Technology · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeCorneal transplantation can treat corneal endothelial diseases. Implanting cultivated human corneal endothelial cells (HCECs) via a cell carrier has clinical value as an alternative therapeutic strategy. This study was performed to compare the feasibility of fish scales and other biomaterials (gelatin and chitosan) as cell carriers and to investigate the effects of an extracellular matrix (ECM) protein coating to improve the cytocompatibility of fish scales.

methodsThe physical properties of gelatin, chitosan, and fish scales were compared. Immortalized HCECs (B4G12) were cultured on processed fish scales, which were coated with fibronectin, laminin, collagen IV, or FNC Coating Mix. Cell attachment and proliferation were evaluated by immunofluorescence, cell counting, and bromodeoxyuridine (BrdU) labeling assays. Immunoblots were used to examine the expression levels of integrin-linked kinase (ILK), phosphate-ILK, β-catenin, p63, and cell cycle mediators (cyclin D1 and p27

resultsThe transparency of processed fish scales was better than that of chitosan, while the strength was higher than that of gelatin. The laminin, collagen IV, and FNC coatings facilitated B4G12 cell adhesion and proliferation, while fibronectin only facilitated cell adhesion. The laminin, collagen IV, and FNC coatings also upregulated phosphate-ILK and p63 expression. In addition, the FNC coating activated cell cycle mediators.

conclusionECM protein-coated processed fish scales can serve as a novel cell carrier to facilitate the development of HCEC transplantation. TRANSLATIONAL RELEVANCE: Improving the physical properties and cytocompatibility of fish scales as a cell carrier will facilitate the transplantation of HCECs into corneas for the purpose of cell therapy.

Indexed as

cytocompatibilityextracellular matrix proteinfish scaleshuman corneal endothelial cellssurface coating

Identifiers

PMID31171994
PMCPMC6543859
OpenAlexW2946910918

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.