ArticlePlant methods2019
A phenol/chloroform-free method to extract nucleic acids from recalcitrant, woody tropical species for gene expression and sequencing.
Article in Plant methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 109 citations in OpenAlex.
- Comparative whole genome analysis of Mycobacterium tuberculosis isolated from BPaLM and individualized regimen treated patients at Dr. Soetomo Hospital, Indonesia.Molecular biology reports · 2026Article
- Optimized Method for Efficient DNA Extraction from Agricultural Soils.Methods and protocols · 2026Article
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- Multi-omics reveal the gut microbiota-mediated severe foraging environment adaption of small wild ruminants in the Three-River-Source National Park, China.Integrative zoology · 2025Article
- Exploring Different Roles ofLife (Basel, Switzerland) · 2025Article
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- Zwitterionic Poly(sulfobetaine methacrylate) Brushes Functionalized Threads for DNA Extraction from Complex Cell Lysates.Sensors (Basel, Switzerland) · 2025Article
- Exogenous diatoms ameliorate thermal bleaching of symbiont bearing benthic foraminifera.Proceedings. Biological sciences · 2025Article
- Duckweed genomes and epigenomes underlie triploid hybridization and clonal reproduction.Current biology : CB · 2025Article
- Loss-of-function mutations in the fruit softening geneHorticulture research · 2025Article
- Optimized protocol for culturing and extracting DNA from fungal isolates associated with brown spot needle blight in pine trees.PloS one · 2025Article
- Effects of monocropping soil on plant growth and rhizosphere microbial community structure ofPeerJ · 2025Article
- Total RNA Extraction from Rice Vegetative Tissues Using Magnetic Beads.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Characterization of the PaHAK Gene and Its Expression During the In Vitro Seed Germination of Two Botanical Avocado Varieties Under Saline Stress.Life (Basel, Switzerland) · 2024Article
- AG1Current issues in molecular biology · 2024Article
- Article
- Rare but diverse off-target and somatic mutations found in field and greenhouse grown trees expressing CRISPR/Cas9.Frontiers in bioengineering and biotechnology · 2024Article
- Research Progress of Nucleic Acid Detection Technology for Genetically Modified Maize.International journal of molecular sciences · 2023Review
- Molecular Cues for Phenological Events in the Flowering Cycle in Avocado.Plants (Basel, Switzerland) · 2023Article
- Auxin-independent effects of apical dominance induce changes in phytohormones correlated with bud outgrowth.Plant physiology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWoody tropical plants contain high levels of complex organic compounds that inhibit the chemical procedures needed to extract RNA or DNA, thus compromising downstream applications such as RNA sequencing and analysis of gene expression. To overcome this issue, researchers must use extraction protocols using CTAB/PVP buffer instead of commercially available DNA/RNA extraction kits. However, these protocols are time-consuming, use toxic chemicals like phenol and chloroform, and can only be used to process a small number of samples at a time. To overcome these issues, we developed a new CTAB/PVP based protocol for RNA or DNA extraction that eliminates the traditional phenol/chloroform step. Furthermore, the protocol was developed for 96-well plates to speed up processing.
resultsOur new protocol enabled us to successfully extract RNA from macadamia, avocado, and mango tissues that are traditionally difficult to work with. This RNA was then successfully used to synthesise cDNA for real-time quantitative PCR and to generate good quality RNA-Seq libraries. Our protocol can be easily converted for rapid DNA extraction from different tropical and sub-tropical tree species.
conclusionThis method enables safer and faster DNA and RNA extraction from recalcitrant species, thus facilitating future work on tropical trees.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.