ArticleScientific reports2019
Filling the gap between collection, transport and storage of the human gut microbiota.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 3 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Cystic Fibrosis-Related Gut Dysbiosis: A Systematic Review.Digestive diseases and sciences · 2023Pooled it
- A systematic review of gut microbiota composition in observational studies of major depressive disorder, bipolar disorder and schizophrenia.Molecular psychiatry · 2022Pooled it
- Probiotic Strains and Intervention Total Doses for Modulating Obesity-Related Microbiota Dysbiosis: A Systematic Review and Meta-analysis.Nutrients · 2020Pooled it
- Next Generation Probiotics Challenges: A Road to use as Live Bio-Therapeutics.Current microbiology · 2026Review
- Toward standardized methods in canine vaginal microbiome research: evaluation of storage, host DNA depletion, and database selection.Microbiology spectrum · 2025Article
- Development of an infant colon simulatingMicrobiology spectrum · 2024Article
- Next-generation IgA-SEQ allows for high-throughput, anaerobic, and metagenomic assessment of IgA-coated bacteria.Microbiome · 2024Article
- Frequency and genotyping of group A rotavirus among Egyptian children with acute gastroenteritis: a hospital-based cross-sectional study.Virology journal · 2024Article
- Protocol for the collection, packaging, and transportation of preterm newborn stool samples.Clinics (Sao Paulo, Brazil) · 2024Observational
- Knowledge and perceptions of South African blood donors towards biobanking and stool donation.Southern African journal of infectious diseases · 2024Article
- Article
- Microbes for lung cancer detection: feasibility and limitations.Frontiers in oncology · 2024Review
- Development of a Protocol for Anaerobic Preparation and Banking of Fecal Microbiota Transplantation Material: Evaluation of Bacterial Richness in the Cultivated Fraction.Microorganisms · 2023Article
- Validation of a batch cultivation protocol for fecal microbiota of Kenyan infants.BMC microbiology · 2023Article
- Comparison of DNA extraction methods for 16S rRNA gene sequencing in the analysis of the human gut microbiome.Scientific reports · 2023Article
- Article
- GutAliveFrontiers in microbiology · 2023Article
- BIOME-Preserve: A novel storage and transport medium for preserving anaerobic microbiota samples for culture recovery.PloS one · 2022Article
- Parkinson's disease patients' short chain fatty acids production capacity after in vitro fecal fiber fermentation.NPJ Parkinson's disease · 2021Article
- Technological tools and strategies for culturing human gut microbiota in engineered in vitro models.Biotechnology and bioengineering · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stool collection devices minimizing the exposure of gut bacteria to oxygen are critical for the standardization of further microbiota-based studies, analysis and developments. The aim of this work was to evidence that keeping anaerobiosis has a deep impact on the viability and diversity of the fecal microbiota that is recovered in the laboratory. Recovering certain microbial populations, such as obligate anaerobic bacteria, is particularly critical if the purpose of the study is to envisage personalized therapeutic purposes, such as autologous Fecal Microbiota Transplant. In this study the same fecal specimens were sampled in conventional stool containers and GutAlive, a disposable device that minimizes exposure of the gut microbiota to oxygen. Samples from five healthy donors were analysed and 150 differential colonies were recovered and identified by 16S rRNA gene sequencing. Globally, GutAlive maintained extremely oxygen sensitive (EOS) populations that were lost in conventional stool containers, and thus viability of species such as as Akkermansia muciniphila, Faecalibacterium prausnitzii and a novel member of the Clostridiales order was kept. These obligate anaerobes were not recovered using the conventional stool collection device. In conclusion, the use of GutAlive for stool collection and transport optimized the viability and recovery of EOS bacteria in the lab by diminishing oxygen toxicity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.