ArticleScientific reports2019
Systematic assessment of antibody selectivity in plasma based on a resource of enrichment profiles.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
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- Review
- Elevated plasma complement factor H related 5 protein is associated with venous thromboembolism.Nature communications · 2023Article
- Multiplexed selectivity screening of anti-GPCR antibodies.Science advances · 2023Article
- Surface protein profiling of prostate-derived extracellular vesicles by mass spectrometry and proximity assays.Communications biology · 2022Article
- Lipid Profiles of Human Serum Fractions Enhanced with CD9 Antibody-Immobilized Magnetic Beads.Metabolites · 2022Article
- Proteomics in thrombosis research.Research and practice in thrombosis and haemostasis · 2022Article
- Identification of Endothelial Proteins in Plasma Associated With Cardiovascular Risk Factors.Arteriosclerosis, thrombosis, and vascular biology · 2021Article
- An artificial neural network approach integrating plasma proteomics and genetic data identifies PLXNA4 as a new susceptibility locus for pulmonary embolism.Scientific reports · 2021Article
- Genetics meets proteomics: perspectives for large population-based studies.Nature reviews. Genetics · 2021Review
- Affinity Proteomics Assays for Cardiovascular and Atherosclerotic Disease Biomarkers.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Bead-Based Assays for Validating Proteomic Profiles in Body Fluids.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Enhanced Validation of Antibodies Enables the Discovery of Missing Proteins.Journal of proteome research · 2020Article
- Liquid biopsy technologies based on membrane microfluidics: High-yield purification and selective quantification of biomarkers in nanocarriers.Electrophoresis · 2020Review
- Facets of individual-specific health signatures determined from longitudinal plasma proteome profiling.EBioMedicine · 2020Article
- Progress on Identifying and Characterizing the Human Proteome: 2019 Metrics from the HUPO Human Proteome Project.Journal of proteome research · 2019Article
- Mass Spectrometry-Based Plasma Proteomics: Considerations from Sample Collection to Achieving Translational Data.Journal of proteome research · 2019Review
- Current applications of antibody microarrays.Clinical proteomics · 2018Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is a strong need for procedures that enable context and application dependent validation of antibodies. Here, we applied a magnetic bead assisted workflow and immunoprecipitation mass spectrometry (IP-MS/MS) to assess antibody selectivity for the detection of proteins in human plasma. A resource was built on 414 IP experiments using 157 antibodies (targeting 120 unique proteins) in assays with heat-treated or untreated EDTA plasma. For each protein we determined their antibody related degrees of enrichment using z-scores and their frequencies of identification across all IP assays. Out of 1,313 unique endogenous proteins, 426 proteins (33%) were detected in >20% of IPs, and these background components were mainly comprised of proteins from the complement system. For 45% (70/157) of the tested antibodies, the expected target proteins were enriched (z-score ≥ 3). Among these 70 antibodies, 59 (84%) co-enriched other proteins beside the intended target and mainly due to sequence homology or protein abundance. We also detected protein interactions in plasma, and for IGFBP2 confirmed these using several antibodies and sandwich immunoassays. The protein enrichment data with plasma provide a very useful and yet lacking resource for the assessment of antibody selectivity. Our insights will contribute to a more informed use of affinity reagents for plasma proteomics assays.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.