ArticleMicrobial cell factories2019
Reconstruction of a Genome Scale Metabolic Model of the polyhydroxybutyrate producing methanotroph Methylocystis parvus OBBP.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Leveraging genome-scale metabolic models to understand aerobic methanotrophs.The ISME journal · 2024Pooled it
- Molecular insights into atmospheric methane-oxidizing USCγ from desert grassland soil based on metagenome-assembled genome analysis.ISME communications · 2026Article
- Linking microbes toFrontiers in microbiology · 2026Article
- Biological conversion of methane to organic molecules: towards a low-carbon bioeconomy.National science review · 2026Review
- Non-growth substrate ethane perturbs core methanotrophy in obligate methanotrophApplied and environmental microbiology · 2025Article
- Review
- Direct Methane Oxidation by Copper- and Iron-Dependent Methane Monooxygenases.Chemical reviews · 2024Review
- Genome-scale flux balance analysis reveals redox trade-offs in the metabolism of the thermoacidophileFrontiers in systems biology · 2024Article
- State-of the-Art Constraint-Based Modeling of Microbial Metabolism: From Basics to Context-Specific Models with a Focus on Methanotrophs.Microorganisms · 2023Review
- A Complete Genome of the Alphaproteobacterial Methanotroph Methylocystis parvus OBBP.Microbiology resource announcements · 2023Article
- Comparative genomic analysis of Methylocystis sp. MJC1 as a platform strain for polyhydroxybutyrate biosynthesis.PloS one · 2023Article
- Article
- Systems Metabolic Engineering of Methanotrophic Bacteria for Biological Conversion of Methane to Value-Added Compounds.Advances in biochemical engineering/biotechnology · 2022Article
- Imaging and modelling of poly(3-hydroxybutyrate) synthesis in Paracoccus denitrificans.AMB Express · 2021Article
- Biochemistry of aerobic biological methane oxidation.Chemical Society reviews · 2021Review
- Strategies for EnhancingFrontiers in bioengineering and biotechnology · 2021Article
- Genomic and Physiological Properties of a Facultative Methane-Oxidizing Bacterial Strain ofMicroorganisms · 2020Article
- Genome Scale Metabolic Model of the versatile methanotroph Methylocella silvestris.Microbial cell factories · 2020Article
- Understanding the metabolism of the tetralin degrader Sphingopyxis granuli strain TFA through genome-scale metabolic modelling.Scientific reports · 2020Article
- Genome-Scale Metabolic Model Reconstruction and in Silico Investigations of Methane Metabolism inMicroorganisms · 2020Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundMethylocystis parvus is a type II methanotroph characterized by its high specific methane degradation rate (compared to other methanotrophs of the same family) and its ability to accumulate up to 50% of its biomass in the form of poly-3-hydroxybutyrate (PHB) under nitrogen limiting conditions. This makes it a very promising cell factory.
resultsThis article reports the first Genome Scale Metabolic Model of M. parvus OBBP. The model is compared to Genome Scale Metabolic Models of the closely related methanotrophs Methylocystis hirsuta and Methylocystis sp. SC2. Using the reconstructed model, it was possible to predict the biomass yield of M. parvus on methane. The prediction was consistent with the observed experimental yield, under the assumption of the so called "redox arm mechanism" for methane oxidation. The co-consumption of stored PHB and methane was also modeled, leading to accurate predictions of biomass yields and oxygen consumption rates and revealing an anaplerotic role of PHB degradation. Finally, the model revealed that anoxic PHB consumption has to be coupled to denitrification, as no fermentation of PHB is allowed by the reconstructed metabolic model.
conclusionsThe "redox arm" mechanism appears to be a general characteristic of type II methanotrophs, versus type I methanotrophs that use the "direct coupling" mechanism. The co-consumption of stored PHB and methane was predicted to play an anaplerotic role replenishing the serine cycle with glyoxylate and the TCA cycle with succinyl-CoA, which allows the withdrawal of metabolic precursors for biosynthesis. The stored PHB can be also used as an energy source under anoxic conditions when coupled to denitrification.
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