Evidence map›Paper›PMID 31170468›Full record

ArticleJournal of virological methods2019

Development and Evaluation of a One-Step Quantitative RT-PCR Assay for Detection of Lassa Virus.

Vladimir G Dedkov, N 'Faly Magassouba, Marina V Safonova, Ekaterina V Naydenova, Andrey A Ayginin, Barre Soropogui, Fode Kourouma, Amara B Camara, Jacob Camara, Andrey A Kritzkiy and 3 more

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virological methods, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 2 countries.

Vladimir G DedkovPasteur Institute, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saint-Petersburg, Russia; Martsinovsky Institute of Medical Parasitology, Tropical and Vector Borne Diseases, Sechenov First Moscow State Medical University, Moscow, Russia. Electronic address: vgdedkov@yandex.ru.
N 'Faly MagassoubaLaboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea.
Marina V SafonovaAnti-Plague Center, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia.
Ekaterina V NaydenovaRussian Research Anti-Plague Institute «Microbe», Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saratov, Russia.
Andrey A AygininCentral Research Institute for Epidemiology, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia.
Barre SoropoguiLaboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea.
Fode KouroumaLaboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea.
Amara B CamaraLaboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea.
Jacob CamaraLaboratorie de Virologic B1568, Université Gamal Abdel Nasser de Conakry, Projet de Recherche sur les Fièvres Hémorragiques en Guinée, Conakry, Guinea.
Andrey A KritzkiyRussian Research Anti-Plague Institute «Microbe», Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saratov, Russia.
Igor V TuchkovRussian Research Anti-Plague Institute «Microbe», Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Saratov, Russia.
Mikhail Yu ShchelkanovFar Eastern Federal University, Vladivostok, Russia.
Victor V MaleevCentral Research Institute for Epidemiology, Federal Service on Consumers' Rights Protection and Human Well-Being Surveillance, Moscow, Russia.
Gamal Abdel Nasser University of Conakry · GNCentral Research Institute of Epidemiology · RUFederal Service for Surveillance on Consumer Rights Protection and Human Wellbeing · RUFar Eastern Federal University · RU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lassa fever is a severe viral hemorrhagic illness caused by Lassa virus. Based on estimates, the number of LASV infections ranges from 300,000 to 500,000 cases in endemic areas with a fatality rate of 1%. Development of fast and sensitive tools for the control and prevention of Lassa virus infection as well as for clinical diagnostics of Lassa fever are crucial. Here we reported development and evaluation of a one-step quantitative RT-qPCR assay for the Lassa virus detection - LASV-Fl. This assay is suitable for the detection of lineages I-IV of Lassa virus. The limit of detection of the assay ranged from 10

Indexed as

AdolescentAdultAnimalsChildDNA PrimersDNA ProbesFemaleGuineaHumansLassa FeverLassa virusLimit of DetectionMaleMiddle AgedMurinaeReal-Time Polymerase Chain ReactionDNA PrimersDNA ProbesRNA, ViralGuineaLassa feverLassa virusquantitative RT-PCR

Identifiers

PMID31170468
PMCPMC7113850
OpenAlexW2948614539

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.