ArticleNucleic acids research2019
Crystal structures of a natural DNA polymerase that functions as an XNA reverse transcriptase.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- DNA polymerase I: structure, activity, and function in bacterial DNA replication and repair.Critical reviews in biochemistry and molecular biology · 2026Review
- Rapid evolution of a highly efficient RNA polymerase by homologous recombination.Nature chemical biology · 2026Article
- The XNA alphabet.Nucleic acids research · 2025Review
- Measuring XNA polymerase fidelity in a hydrogel particle format.Nucleic acids research · 2025Article
- Structural insights into a DNA polymerase reading the xeno nucleic acid HNA.Nucleic acids research · 2025Article
- DNA Replication across α-l-(3'-2')-Threofuranosyl Nucleotides Mediated by Human DNA Polymerase η.Biochemistry · 2024Article
- Detection of Specific RNA Targets by Multimerization.Biochemistry. Biokhimiia · 2023Article
- Thermophilic Nucleic Acid Polymerases and Their Application in Xenobiology.International journal of molecular sciences · 2022Review
- Crystal structure of DNA polymerase I from Thermus phage G20c.Acta crystallographica. Section D, Structural biology · 2022Article
- Crystallographic analysis of engineered polymerases synthesizing phosphonomethylthreosyl nucleic acid.Nucleic acids research · 2022Article
- Loop-Mediated Isothermal Amplification: From Theory to Practice.Russian journal of bioorganic chemistry · 2022Article
- One-Enzyme Reverse Transcription qPCR Using Taq DNA Polymerase.Biochemistry · 2020Article
- Structural Studies of HNA Substrate Specificity in Mutants of an Archaeal DNA Polymerase Obtained by Directed Evolution.Biomolecules · 2020Article
- Modified nucleoside triphosphates in bacterial research forRSC chemical biology · 2020Article
- 2'-fluoro-modified pyrimidines enhance affinity of RNA oligonucleotides to HIV-1 reverse transcriptase.RNA (New York, N.Y.) · 2020Article
- In Vitro Selection of an ATP-Binding TNA Aptamer.Molecules (Basel, Switzerland) · 2020Article
- Review
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4 authors.
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Abstract
Replicative DNA polymerases are highly efficient enzymes that maintain stringent geometric control over shape and orientation of the template and incoming nucleoside triphosphate. In a surprising twist to this paradigm, a naturally occurring bacterial DNA polymerase I member isolated from Geobacillus stearothermophilus (Bst) exhibits an innate ability to reverse transcribe RNA and other synthetic congeners (XNAs) into DNA. This observation raises the interesting question of how a replicative DNA polymerase is able to recognize templates of diverse chemical composition. Here, we present crystal structures of natural Bst DNA polymerase that capture the post-translocated product of DNA synthesis on templates composed entirely of 2'-deoxy-2'-fluoro-β-d-arabino nucleic acid (FANA) and α-l-threofuranosyl nucleic acid (TNA). Analysis of the enzyme active site reveals the importance of structural plasticity as a possible mechanism for XNA-dependent DNA synthesis and provides insights into the construction of variants with improved activity.
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