Evidence map›Paper›PMID 31169949›Full record

ArticleJournal of clinical laboratory analysis2019

Expression profiles of circular RNAs in human colorectal cancer based on RNA deep sequencing.

Jiaxin Ge, Yanping Jin, Xueyou Lv, Qi Liao, Cong Luo, Guoliang Ye, Xinjun Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
2.3field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jiaxin GeDepartment of Gastroenterology, The Affiliated Hospital of Medical School, Ningbo University, Ningbo, China.ORCID https://orcid.org/0000-0002-4332-7703
Yanping JinDepartment of Gastroenterology, The Affiliated Hospital of Medical School, Ningbo University, Ningbo, China.
Xueyou LvDepartment of Gastroenterology, The Affiliated Hospital of Medical School, Ningbo University, Ningbo, China.
Qi LiaoDepartment of Biochemistry and Molecular Biology and Zhejiang Key Laboratory of Pathophysiology, Ningbo University School of Medicine, Ningbo, China.
Cong LuoDepartment of Abdominal Oncology, Zhejiang Cancer Hospital, Hangzhou, China.
Guoliang YeDepartment of Gastroenterology, The Affiliated Hospital of Medical School, Ningbo University, Ningbo, China.
Xinjun ZhangDepartment of Gastroenterology, The Affiliated Hospital of Medical School, Ningbo University, Ningbo, China.
Ningbo University · CNZhejiang Cancer Hospital · CN

Funding

K.C. Wong Magna Fund in Ningbo UniversityNatural Science Foundation of Ningbo 2016A610121The Scientific Innovation Team Project of Ningbo 2017C110019
6 · The paper itself

Abstract

backgroundCircular RNAs (circRNAs) are a novel group of RNAs and play essential roles in cancers. However, the expression profiles of circRNAs in human colorectal cancer (CRC) are largely unclear.

methodsThe differentially expressed circRNAs, mRNAs, and microRNAs (miRNAs) between CRC tissues and paired adjacent normal tissues were first screened. Then, gene ontology and pathway analyses were performed to predict the possible functions. In addition, we identified the differentially expressed circRNAs in CRC correlated with Krüppel-like factor 4 (KLF4) and validated their expression levels in CRC tissues. Finally, the correlations between hsa_circ_0142527 expression levels and clinicopathological features of patients with CRC were also analyzed.

resultsAfter filtered 4735 circRNAs by RNA deep sequencing, 67 differentially expressed circRNAs (fold change >2.0, P < 0.05) were selected. The top two pathways were cell cycle and other glycan degradation. Hsa_circ_0142527 and KLF4 mRNA were significantly lower expressed in CRC tissues in both training and confirm groups and have high positive correlation (r = 0.754). We further found that the expression levels of hsa_circ_0142527 were significantly associated with age (P = 0.004), differentiation (P = 0.008), invasion (P = 0.029), distal metastasis (P = 0.004), TNM stage (P = 0.005), and carcinoembryonic antigen (CEA; P = 0.037).

conclusionsThe circRNA expression profile of CRC provided new clues for understanding the occurrence of CRC. Hsa_circ_0142527 may be served as a potential biomarker for the diagnosis of CRC.

Indexed as

Gene Expression ProfilingGene Expression Regulation, NeoplasticHigh-Throughput Nucleotide SequencingSequence Analysis, RNABase SequenceColorectal NeoplasmsDown-RegulationFemaleGene OntologyHumansKruppel-Like Factor 4Kruppel-Like Transcription FactorsMaleMiddle AgedReproducibility of ResultsRNA, CircularKLF4 protein, humanKruppel-Like Factor 4Kruppel-Like Transcription FactorsRNA, CircularRNA, Messengercircular RNAcolorectal cancerhsa_circ_0142527KLF4RNA deep sequencing

Identifiers

PMID31169949
PMCPMC6757124
OpenAlexW2954602611

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.