Evidence map›Paper›PMID 31168087›Full record

ArticleNature protocols2019

Wireless nanopore electrodes for analysis of single entities.

Rui Gao, Yao Lin, Yi-Lun Ying, Yong-Xu Hu, Su-Wen Xu, Lin-Qi Ruan, Ru-Jia Yu, Yuan-Jie Li, Hao-Wen Li, Ling-Fei Cui and 1 more

Abstract read
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In one paragraph

Article in Nature protocols, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Rui GaoSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
Yao LinSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
Yi-Lun YingSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China. yilunying@ecust.edu.cn.ORCID http://orcid.org/0000-0001-6217-256X
Yong-Xu HuSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
Su-Wen XuSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
Lin-Qi RuanSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
Ru-Jia YuSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
Yuan-Jie LiSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
Hao-Wen LiSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
Ling-Fei CuiSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.
Yi-Tao LongSchool of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Measurements of a single entity underpin knowledge of the heterogeneity and stochastics in the behavior of molecules, nanoparticles, and cells. Electrochemistry provides a direct and fast method to analyze single entities as it probes electron/charge-transfer processes. However, a highly reproducible electrochemical-sensing nanointerface is often hard to fabricate because of a lack of control of the fabrication processes at the nanoscale. In comparison with conventional micro/nanoelectrodes with a metal wire inside, we present a general and easily implemented protocol that describes how to fabricate and use a wireless nanopore electrode (WNE). Nanoscale metal deposition occurs at the tip of the nanopipette, providing an electroactive sensing interface. The WNEs utilize a dynamic ionic flow instead of a metal wire to sense the interfacial redox process. WNEs provide a highly controllable interface with a 30- to 200-nm diameter. This protocol presents the construction and characterization of two types of WNEs-the open-type WNE and closed-type WNE-which can be used to achieve reproducible electrochemical measurements of single entities. Combined with the related signal amplification mechanisms, we also describe how WNEs can be used to detect single redox molecules/ions, analyze the metabolism of single cells, and discriminate single nanoparticles in a mixture. This protocol is broadly applicable to studies of living cells, nanomaterials, and sensors at the single-entity level. The total time required to complete the protocol is ~10-18 h. Each WNE costs ~$1-$3.

Indexed as

ElectrodesNanoporesElectrochemical TechniquesEquipment DesignHumansMCF-7 CellsNanoparticlesNanotechnologyOxidation-ReductionPatch-Clamp TechniquesSingle-Cell AnalysisWireless Technology

Identifiers

PMID31168087

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.