ArticlePLoS neglected tropical diseases2019
Aedes aegypti microRNA, miR-2944b-5p interacts with 3'UTR of chikungunya virus and cellular target vps-13 to regulate viral replication.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 32 citations in OpenAlex.
- The 3'Untranslated region is a critical determinant of Getah virus replication, pathogenesis, and vector competence.Virulence · 2026Article
- Dysregulation of miRNAs has broad impacts on virus infection inJournal of virology · 2026Article
- Dual regulatory role of hsa-miR-122b-5p in chikungunya virus infection via interaction with CHIKV 3'-UTR and HDAC4 modulation.Journal of virology · 2025Article
- Identification of miRNAs Involved in Olfactory Regulation in Antennae of Beet Webworm,Life (Basel, Switzerland) · 2024Article
- MicroRNA-22-3p displaces critical host factors from the 5' UTR and inhibits the translation of Coxsackievirus B3 RNA.Journal of virology · 2024Article
- Aal-circRNA-407 regulates ovarian development of Aedes albopictus, a major arbovirus vector, via the miR-9a-5p/Foxl axis.PLoS pathogens · 2023Article
- Identification of MicroRNAs in the West Nile Virus Vector Culex tarsalis (Diptera: Culicidae).Journal of medical entomology · 2023Article
- Review
- Analysis of Aedes aegypti microRNAs in response to Wolbachia wAlbB infection and their potential role in mosquito longevity.Scientific reports · 2022Article
- Interaction between endogenous microRNAs and virus-derived small RNAs controls viral replication in insect vectors.PLoS pathogens · 2022Article
- An E3 Ubiquitin Ligase Scaffolding Protein Is Proviral during Chikungunya Virus Infection in Aedes aegypti.Microbiology spectrum · 2022Article
- Intron-derived small RNAs for silencing viral RNAs in mosquito cells.PLoS neglected tropical diseases · 2022Article
- The Influence of Host miRNA Binding to RNA Within RNA Viruses on Virus Multiplication.Frontiers in cellular and infection microbiology · 2022Review
- Coordination between terminal variation of the viral genome and insect microRNAs regulates rice stripe virus replication in insect vectors.PLoS pathogens · 2021Article
- Review
- Intracellular Interactions Between Arboviruses andFrontiers in cellular and infection microbiology · 2021Review
- Development of miRNA-Based Approaches to Explore the Interruption of Mosquito-Borne Disease Transmission.Frontiers in cellular and infection microbiology · 2021Review
- Profiling of MicroRNAs in Midguts ofFrontiers in genetics · 2021Article
- Review
- Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRNA interference is among the most important mechanisms that serve to restrict virus replication within mosquitoes, where microRNAs (miRNAs) are important in regulating viral replication and cellular functions. These miRNAs function by binding to complementary sequences mostly in the untranslated regions of the target. Chikungunya virus (CHIKV) genome consists of two open reading frames flanked by 5' and 3' untranslated regions on the two sides. A recent study from our laboratory has shown that Aedes miRNAs are regulated during CHIKV infection. The present study was undertaken to further understand the role of these miRNAs in CHIKV replication. METHODS/
findingsWe observe that miR-2944b-5p binds to the 3' untranslated region of CHIKV and the binding is abated when the binding sites are abolished. Loss-of-function studies of miR-2944b-5p using antagomirs, both in vitro and in vivo, reveal an increase in CHIKV viral replication, thereby directly implying a role of miR-2944b-5p in CHIKV replication. We further showed that the mitochondrial membrane potential of the mosquito cells is maintained by this miRNA during CHIKV replication, and cellular factor vps-13 plays a contributing role.
conclusionsOur study has opened new avenues to understand vector-virus interactions and provides novel insights into CHIKV replication in Aedes aegypti. Furthermore, our study has shown miR-2944b-5p to be playing role, where one of its target vps-13 also contributes, in maintaining mitochondrial membrane potential in Aedes aegypti.
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