Evidence map›Paper›PMID 31166131›Full record

ArticleEnvironmental health perspectives2019

Influence of Nanomolar Deltamethrin on the Hallmarks of Primary Cultured Cortical Neuronal Network and the Role of Ryanodine Receptors.

Jing Zheng, Yiyi Yu, Wei Feng, Jing Li, Ju Liu, Chunlei Zhang, Yao Dong, Isaac N Pessah, Zhengyu Cao

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Environmental health perspectives, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
4.4field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 27 citations in OpenAlex.

  1. Article
  2. Marine and Anthropogenic Bromopyrroles Alter Cellular CaEnvironmental science & technology · 2021
    Article
  3. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Jing Zheng1 State Key Laboratory of Natural Medicines, Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, Department of TCM Pharmacology, School of Traditional Pharmacy, China Pharmaceutical University , Nanjing, China.
Yiyi Yu1 State Key Laboratory of Natural Medicines, Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, Department of TCM Pharmacology, School of Traditional Pharmacy, China Pharmaceutical University , Nanjing, China.
Wei Feng2 Department of Molecular Biosciences, School of Veterinary Medicine, University of California , Davis, California, USA.
Jing Li1 State Key Laboratory of Natural Medicines, Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, Department of TCM Pharmacology, School of Traditional Pharmacy, China Pharmaceutical University , Nanjing, China.
Ju Liu1 State Key Laboratory of Natural Medicines, Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, Department of TCM Pharmacology, School of Traditional Pharmacy, China Pharmaceutical University , Nanjing, China.
Chunlei Zhang1 State Key Laboratory of Natural Medicines, Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, Department of TCM Pharmacology, School of Traditional Pharmacy, China Pharmaceutical University , Nanjing, China.
Yao Dong2 Department of Molecular Biosciences, School of Veterinary Medicine, University of California , Davis, California, USA.
Isaac N Pessah2 Department of Molecular Biosciences, School of Veterinary Medicine, University of California , Davis, California, USA.
Zhengyu Cao1 State Key Laboratory of Natural Medicines, Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Research, Department of TCM Pharmacology, School of Traditional Pharmacy, China Pharmaceutical University , Nanjing, China.
China Pharmaceutical University · CNUniversity of California, Davis · US

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI HAMMOCK, BRUCE D · 1987 to 2021
$50.1M
UC Davis Center for Children's Environmental Health (CCEH)P01ES011269 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI VAN DE WATER, JUDY A. · 2001 to 2018
$12.5M
Molecular and Cellular Basis of PCB Developmental Neurotoxicity: ViCTER supplementR01ES014901 · NIEHS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI HANS-JOACHIM LEHMLER, Pamela J Lein · 2009 to 2026
$9.3M
NIEHS NIH HHS P01 ES011269NIEHS NIH HHS P42 ES004699NIEHS NIH HHS R01 ES014901
6 · The paper itself

Abstract

backgroundThe pyrethroid deltamethrin (DM) is broadly used for insect control. Although DM hyperexcites neuronal networks by delaying inactivation of axonal voltage-dependent [Formula: see text] channels, this mechanism is unlikely to mediate neurotoxicity at lower exposure levels during critical perinatal periods in mammals.

objectivesWe aimed to identify mechanisms by which acute and subchronic DM altered axonal and dendritic growth, patterns of synchronous [Formula: see text] oscillations (SCOs), and electrical spike activity (ESA) functions critical to neuronal network formation.

methodsMeasurements of SCOs using [Formula: see text] imaging, ESA using microelectrode array (MEA) technology, and dendritic complexity using Sholl analysis were performed in primary murine cortical neurons from wild-type (WT) and/or ryanodine receptor 1 ([Formula: see text]) mice between 5 and 14 d in vitro (DIV). [Formula: see text] binding analysis and a single-channel voltage clamp were utilized to measure engagement of RyRs as a direct target of DM.

resultsNeuronal networks responded to DM ([Formula: see text]) as early as 5 DIV, reducing SCO amplitude and depressing ESA and burst frequencies by 60-70%. DM ([Formula: see text]) enhanced axonal growth in a nonmonotonic manner. [Formula: see text] enhanced dendritic complexity. DM stabilized channel open states of RyR1, RyR2, and cortical preparations expressing all three isoforms. DM ([Formula: see text]) altered gating kinetics of RyR1 channels, increasing mean open time, decreasing mean closed time, and thereby enhancing overall open probability. SCO patterns from cortical networks expressing [Formula: see text] were more responsive to DM than WT. [Formula: see text] neurons showed inherently longer axonal lengths than WT neurons and maintained less length-promoting responses to nanomolar DM.

conclusionsOur findings suggested that RyRs were sensitive molecular targets of DM with functional consequences likely relevant for mediating abnormal neuronal network connectivity in vitro. https://doi.org/10.1289/EHP4583.

Indexed as

AnimalsAxonsCalciumCells, CulturedFemaleInsecticidesMaleMice, Inbred C57BLMice, KnockoutNeuronal PlasticityNeuronsNitrilesPyrethrinsRyanodine Receptor Calcium Release ChannelCalciumdecamethrinInsecticidesNitrilesPyrethrinsRyanodine Receptor Calcium Release Channel

Identifiers

PMID31166131
PMCPMC6792378
OpenAlexW2948465206

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.